For automated isolation and purification of DNA from liquid-based cytology media
The QIAsymphony DSP AXpH DNA Kit, in combination with the QIAsymphony SP, enables automated DNA purification from liquid-based cytology media for in vitro diagnostic use. Optimized protocols provide DNA eluates that are ready to use in downstream applications. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents. The QIAsymphony SP performs all steps of the sample preparation procedure. Up to 96 samples, in batches of up to 24, are processed in a single run.
The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see " QIAsymphony DSP AXpH procedure"). Worktable setup is rapid, which saves valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, allowing complete cost control.
| Protocol | Sample type | Volume processed |
|---|---|---|
| AXpH2000_DSP | PreservCyt samples | 2 ml |
| SP2000_DSP | Diluted SurePath postgradient samples | 2 ml |
The QIAsymphony DSP AXpH DNA Kit in combination with the QIAsymphony SP enables purification of DNA from liquid-based cytology samples, such as cervical swabs collected in SurePath (BD) or PreservCyt (Cytyc/Hologic) media.
