NGS-Workflows

With the advances brought by next-generation sequencing (NGS), RNA-seq has become critical for a diverse range of studies of the many different types of RNA. Current technologies go well beyond looking at just mRNA expression and include sequencing miRNA as well as entire transcriptomes. Several techniques have been developed recently to overcome key challenges and improve results by, for example, depleting rRNA and by targeting specific sets of transcripts using digital RNA-seq.

rRNA removal

Improve RNA-seq with effective rRNA removal

During RNA-seq library preparation, highly abundant RNAs that are of low scientific value, such as rRNA and/or globin mRNA, can use up valuable sequencing capacity, jeopardizing the ability to achieve on-target gene expression reads. Effective removal of unwanted RNA is a critical step prior to RNA-seq.
The novel QIAseq FastSelect rRNA Removal technology removes unwanted RNAs in a single pipetting step from mammalian, bacterial, plant and even yeast RNA samples. Whether you’re looking to remove cytoplasmic and mitochondrial rRNA for whole transcriptome analysis or get rid of globin mRNA for gene-expression research using blood samples, the new technology can maximize usable reads.

Webinar on next-generation rRNA removal:

The R&D team lead a discussion of how we developed and benchmarked our QIAseq FastSelect rRNA Removal Kits for human, mammalian, bacterial, plant and yeast samples using both high-quality and severely fragmented total RNA.

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Webinar] Next-generation rRNA removal: Evolution of new technologies for mammalian, bacterial, plant and yeast samples

Ribosomal RNA removal: The most underestimated step in RNA-seq optimization

Read the story of Dr. Fabienne Desmots-Loyer, Hospital Rennes, and find out how QIAseq FastSelect saved tby providing high-quality libraries from FFPE cancer samples previously rejected for processing by two commercial labs due to high levels of RNA degradation and low yields.

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Ribosomal RNA removal: The most underestimated step in RNA-seq optimization

QIAseq solutions

QIAseq Stranded RNA Library Kits
QIAseq Stranded mRNA Select Kits include an optimized mRNA enrichment protocol to build high-quality, stranded RNAseq libraries. Combine with QIAseq FastSelect technology for RNA removal prior to stranded library prep for more on-target RNA-seq reads.
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QIAseq FastSelect RNA Removal Kits
Explore the entire QIAseq FastSelect product line. Whether you’re working with mammalian, plant, bacterial or yeast RNA-seq samples, we’ve got a dedicated rRNA removal solution. Also browse through QIAseq FastSelect success stories from the research community.
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QIAseq RNA-solutions for FFPE samples
High levels of degradation and poor yields are a challenge for working with RNA from FFPE samples. Read this application note to learn how low RIN RNA from FFPE samples were rescued for improved RNA sequencing.
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Biomarker discovery

Sample to Insight for optimized miRNA biomarker discovery

QIAseq miRNA sequencing solutions for miRNA, piRNA and small RNA expression analysis, as well as discovery of novel small RNAs, offer small RNA NGS library preparation and quality control of sample preparation and NGS performance.

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Webinar – “The truth about RNA sequencing”

Learn from one of our top experts in the field, Dr. Barbara R. Gould, and the many years of experience gained by QIAGEN Genomic Services. This webinar covers issues including library preparation, sequencing parameters, quality control, data analysis and validating NGS results.

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NGS for microRNA detection

Evaluation of methodologies for microRNA biomarker detection by NGS
Coenen-Stass and colleagues tested commercial kits for small RNA sequencing. Their cohort included human plasma, human serum, mouse brain and a reference library. They provide guidelines for choosing library preparation for sensitivity, specificity and accuracy as well as convenience.
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miRNA NGS Data Analysis Report
QIAGEN Genomic Services provides an miRNA sequencing service. Check out this sample report to see the workflow and the kinds of results you can expect when you use the miRNA NGS Seq Service.
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3’ RNA sequencing

The advantages of 3’ sequencing

The use of 3’ RNA sequencing has been instrumental in cell signaling pathways to meet the increasing need for high-throughput solutions that also ensure increased accuracy, specificity and sensitivity. These user stories explain how they gained high-throughput 3' transcriptome NGS from ultralow amounts of RNA for gene expression analysis.

Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signaling

“For us, the QIAseq UPX 3’ Transcriptome approach was a life-saver, allowing high-throughput sequencing on ultralow amounts of RNA isolated from hard-to-process samples. The pilot results obtained with QIAGEN Genomic Services exceeded our expectations. In my opinion, this is certainly an approach to keep in mind when the RNA quantity is below the minimal threshold.” Simon Chera, Ph.D., Associate Professor, University of Bergen.

Read the Nature publication

Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signaling

Webinar – uncovering the connection

"QIAGEN's UPX 3’ Transcriptome service allowed us to generate a large-scale RNA-seq data set that facilitated our discovery of key gene expression changes that drive our disease model." Matthew Dodson, Ph.D., Post-Doctoral Researcher, University of Arizona.

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[Webinar] Uncovering the connection between arsenic and type II diabetes

Featured products

QIAseq FastSelect RNA Removal Kits
For rapid rRNA removal for RNA-seq library preparation from mammalian, bacterial, plant and yeast RNA-seq samples.
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QIAseq Stranded RNA Library Kits
For preparation of stranded whole transcriptome RNAseq libraries for NGS applications on Illumina instruments.
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QIAseq UPX 3’ Targeted RNA Panels
For ultraplex (UPX) gene expression analysis using 3' RNAseq from single cells and low-input samples.
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Genomic Services

From library prep and sequencing to data analysis and interpretation, our experts are always here to help. With years of experience and trusted technologies, we deliver highquality results you can rely on.

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NGS workflows