QIAEX II Gel Extraction Kit

For purification of DNA fragments (40 bp to 50 kb) from gels and solutions
  • Efficient extraction of DNA from 40 bp to 50 kb
  • Gel extraction from TAE or TBE agarose gels and polyacrylamide gels
  • No sodium iodide to interfere with subsequent reactions
  • No shearing of large DNA fragments
The QIAEX II Gel Extraction Kit provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed, and DNA from 40 bp to 50 kb is eluted in Tris buffer or water.
产品 货号 目录价:
QIAEX II Gel Extraction Kit (150)
For 150 extractions: 3 x 0.5 ml QIAEX II Suspension, Buffers
20021
询价
QIAEX II Gel Extraction Kit (500)
For 500 extractions: 5 x 1.0 ml QIAEX II Suspension, Buffers
20051
询价

QIAEX II Gel Extraction Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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QIAEX II实验流程。|pH Indicator Dye。|回收率稳定。|
|利用溶解液和结合缓冲液中的pH指示剂染料可轻松观测DNA吸附的最佳pH值 (pH ≤7.5)。如果琼脂糖凝胶电泳缓冲液使用频繁或者配制不当,则会出现错误的结合混合物pH值。此时,可加入10 µl 3 M醋酸钠 (pH 5.0) 轻松调整pH值。|使用QIAEX II Gel Extraction Kit,从1%琼脂糖凝胶中回收的不同量的2.9 kb DNA片段如图所示。|
性能

Using the QIAEX II Gel Extraction Kit, 10 ng to 10 µg DNA are recovered efficiently (see figure "Consistent recovery"). The versatile batch procedure can be easily scaled up to a binding capacity of 15 µg using 30 µl QIAEX II suspension.

The QIAEX II Gel Extraction Kit provides silica particles for purifying 60–95% DNA fragments (40 bp – 50 kb). A volume of 10 µl QIAEX II suspension binds up to 5 µg DNA, which is subsequently eluted in 20 µl.

Recovery according to size
DNA sizeRecovery, percent*
44 bp 75
75 bp 75
500 bp 95
7.5 kb 85
23.5 kb 75
48.5 kb 60
* From 2 µg loaded on a 1% TAE agarose gel.
原理

Purification of DNA fragments with the QIAEX II system is based on solubilization of agarose and selective adsorption of nucleic acids onto QIAEX II silica-gel particles in the presence of chaotropic salt. QIAEX II separates DNA from salts, agarose, polyacrylamide, dyes, proteins, and nucleotides without phenol extraction or ethanol precipitation. QIAEX II is effective for any type of agarose in either TAE or TBE buffers.

QIAEX II particles ensure efficient recovery without shearing, even for large DNA fragments. Optimized buffers permit DNA recovery without sodium iodide, which is difficult to remove from DNA samples, and may affect subsequent reactions.

The solubilization and binding buffer used with the QIAEX II system contains a unique pH indicator. A simple color change indicates whether the pH of the binding mixture is optimal for efficient adsorption of DNA to QIAEX II silica particles (see figure "pH indicator dye"). The colored dye also allows easy visualization of any unsolubilized agarose in the binding mixture, ensuring complete solubilization for maximum yield.

操作流程

QIAEX II silica-gel particles are added to the solubilized gel slice, and the particles collected by a brief centrifugation step (see flowchart "QIAEX II procedure"). After washing, the pure DNA fragment is eluted in 20 µl of Tris buffer or water.

The QIAEX II Gel Extraction Kit provides QIAEX II suspension together with binding and wash buffers, and a comprehensive handbook. QIAEX II Suspension is also sold separately. Protocols are provided for purification of DNA from agarose gels, solutions, and polyacrylamide gels.

应用

DNA purified with the QIAEX II system can be used directly in most applications, including:

Restriction digestion
Labeling
Ligation
PCR
特点
参数
结合能力 5 µg/10 µl
洗脱体积 20 µl
规格 Tube
片段大小 40 bp – 50 kb
处理 Manual
回收:寡核苷酸dsDNA Recovery: dsDNA fragments
去除<10mers、17–40mers的染料终止子蛋白 Removal <40mers
样本类型:应用 DNA: PCR reactions
技术 Silica technology

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安全数据表
1
Download Safety Data Sheets for QIAGEN product components.
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图片
QIAEX II Procedure
QIAEX II实验流程。
ILLU_0104_MinElute
pH Indicator Dye。
利用溶解液和结合缓冲液中的pH指示剂染料可轻松观测DNA吸附的最佳pH值 (pH ≤7.5)。如果琼脂糖凝胶电泳缓冲液使用频繁或者配制不当,则会出现错误的结合混合物pH值。此时,可加入10 µl 3 M醋酸钠 (pH 5.0) 轻松调整pH值。
Consistent Recovery from Different Starting Quantities of DNA Fragment
回收率稳定。
使用QIAEX II Gel Extraction Kit,从1%琼脂糖凝胶中回收的不同量的2.9 kb DNA片段如图所示。