MinElute 96 UF PCR Purification Kit

For high-throughput, ultrafiltration purification of up to 15 μg PCR products (≥100 bp)

  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries

The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products ≥100 bp in size. The plates are designed to allow elution in very small volumes (as little as 20 μl) delivering high yields of highly concentrated DNA. The procedure can be automated on the BioRobot Universal System. DNA fragments purified with the MinElute system are ready for direct use in many applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute 96 UF PCR Purification Kit requires the use of the QIAvac Multiwell vacuum manifold (cat. no. 9014579).
The product must be used in conjunction with QIAvac Multiwell.

製品名 Cat. no. List price:
MinElute 96 UF PCR Purification Kit (4)
4 MinElute 96 UF PCR Purification Plates
28051
¥32,500
MinElute 96 UF PCR Purification Kit (24)
24 MinElute 96 UF PCR Purification Plates
28053
¥162,000

MinElute 96 UF PCR Purification Kit  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


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MinElute 96 UF PCR Purification procedure.|Highly concentrated DNA eluates.|
Using the manual procedure, 96 samples are prepared in 18 minutes.
|Efficient purification of concentrated DNA was achieved using the MinElute 96 UF PCR Purification Kit. DNA fragments (100 bp and 500 bp) were purified using either MinElute 96 UF PCR Purification Plates (QIAGEN) or ultrafiltration-based kits from other suppliers (Supplier MIII and Supplier M). Aliquots (5 μl) of the eluate were analyzed by agarose gel electrophoresis. M: markers.|
Performance
The MinElute 96 UF PCR Purification Kit provides high-throughput PCR purification in 96-well plates. Fully automated processing is provided for 1–8 x 96 samples on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System, or for 1 x 96 samples on the QIAvac Multiwell Manifold (cat. no. 9014579, requires suitable vacuum manifold, e.g., QIAvac Multiwell manifold, plate shaker recommended). The procedure purifies DNA ≥100 bp in size with a recovery of 80–95%. Purified PCR products are eluted directly from the surface of the membrane in small volumes (as little as 20 µl in the manual procedure or 30 µl on BioRobot workstations), providing high-concentration eluates (see figure "Highly concentrated DNA eluates") that are suitable for direct use in a variety of downstream applications.  It is possible to process PCR sample volumes up to 150 µl using either a manual or fully automated procedure.
Principle

The QIAGEN MinElute 96 UF PCR Purification Kit combines the convenience of multiwell technology and the separation properties of an ultrafiltration membrane with a unique design. The plates enable the fast and efficient recovery of double-stranded DNA fragments ≥100 bp and the removal of primers (<20mers), unincorporated nucleotides, and salts.

Procedure
MinElute 96 UF PCR Purification Kits provide ultrafiltration technology using vacuum-driven purification on QIAvac manifolds (see flowchart "MinElute 96 UF PCR Purification procedure"). PCR products are loaded into the wells of the ultrafiltration plates, and a vacuum is applied. While small molecules such as primers, salts, and unincorporated nucleotides pass through the membrane, PCR products ≥100 bp are retained. Water, DMSO, or buffers such as SSC can be used for elution. The MinElute 96 UF PCR Purification procedure can also be automated on the BioRobot 3000 (no longer available), the BioRobot 8000, or the BioRobot Universal System.
Applications

DNA fragments purified with the MinElute 96 UF system are ready for direct use in many applications, including:

Sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling

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For efficient, high-throughput purification of PCR products
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PCR産物の効率的なハイスループット精製用
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MinElute 96 UF PCR Purification Procedure
MinElute 96 UF PCR Purification製法
96サンプル、18分間(マニュアル製法)。
Highly Concentrated DNA
高濃度のDNA溶出液
MinElute 96 UF PCR Purification Kitを用いて高濃度なDNAを効率的に精製できた。MinElute 96 UF PCR Purification Plates(QIAGEN)または別のメーカー(Supplier MIII および Supplier M)の限外ろ過法を用いたキットを用いてDNA断片(100 bpおよび500 bp)を精製した。溶出液の5 µlをアガロースゲル電気泳動で解析した。 M:マーカー。