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DNeasy PowerWater Sterivex Kit

For the isolation of genomic DNA from all filtered water sample types collected with Sterivex filter units
  • In-unit digestion saves time and samples
  • Column-based purification protocol eliminates the need for harsh chemicals
  • Fast protocol takes only 40 minutes
Removing important sample data from Millipore’s Sterivex filter units present a unique set of problems for researchers, however the DNeasy PowerWater Sterivex Kit is the only kit available to provide DNA from these filters without enzymes or harsh organic chemicals. Microbes are released from Sterivex filter units utilizing a novel Cell Release Solution that eliminates extensive incubation times or breaking the casing around the membrane. With Inhibitor Removal Technology, high-quality DNA isolation is possible, even when the original sample was highly contaminated. In 40 minutes, DNA can be purified from all types of water samples.

This kit is recommended for use with Millipore catalog # SVGPL10RC Sterivex GP Filter units, but is also compatible with other Sterivex Filter units.

The DNeasy PowerWater Sterivex Kit was previously sold by MO BIO as the PowerWater Sterivex DNA Kit.
Cat No./ID: 14600-50-NF
DNeasy PowerWater Sterivex Kit (50)
kr6,465.00
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For the isolation genomic DNA from water samples collected with Sterivex Filter Units
The DNeasy PowerWater Sterivex Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1.
The protocol for the DNeasy PowerWater Sterivex Kit can be completed in just 40 minutes, including cell lysis via bead based homogenization and DNA purification. Protocols using an in-unit enzymatic digestion followed by a phenol/chloroform extraction require over 20 hours to complete and include long incubations, enzymatic digestion and purification protocols.
1
Figure 2.
DNA isolated using the DNeasy PowerWater Sterivex Kit exhibited a higher yield of high MW, dsDNA with less RNA carryover compared with other common in-unit or out-of-unit lysis protocols followed by phenol/chloroform extraction.
2
Figure 3.
DNA isolated using the DNeasy PowerWater Sterivex Kit is free of PCR inhibitors, while DNA purified using an in-unit lysis protocol followed by phenol/chloroform extraction exhibited PCR inhibition and failed to amplify without a 1:10 dilution.
Procedure
To begin the DNeasy PowerWater Sterivex Kit protocol, Sterivex units are treated and incubated with cell release solution. Units are then treated with lysis buffer, followed by mixing and removing lysate for homogenization in a 5 ml bead beating tube. Total genomic DNA is captured after protein and inhibitor removal steps on a silica binding column using a vacuum manifold. The binding column membrane is then washed, resulting in pure, high-quality DNA being eluted for downstream applications.
Features
Specifications
Bead size 0.1 mm glass
Binding capacity Up to 20 µg per prep
Format Silica Spin Filter Tubes
Processing Bead beating
Sample types Processed ocean water, fresh water, brackish water, ground water, waste water
Storage temperature Store at room temperature (15-30°C)
Throughput 1-6 samples
Time per run or per prep 40 minutes
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