Multi-Analyte ELISArray Kits
For the simultaneous detection of up to 12 cytokines or chemokines in multiple biological samples using an ELISA
Multi-Analyte ELISArray Kits are the simplest simultaneous multi-analyte ELISA available. Highly specific capture antibodies for key cytokines and chemokines are arrayed in a 96-well plate. One easy ELISA protocol allows the detection of 12 cytokines or chemokines simultaneously in multiple samples.
One example of a Multi-Analyte ELISArray Kit application is as follows. A Multi-Analyte ELISArray Kit was used to profile multiple cytokines secreted by stimulated human peripheral blood mononuclear cells (PBMCs). The time-dependent (0, 6, 18, 24, and 48 hours) patterns of 8 Th1/Th2 cytokine inductions by human PBMCs were montored in response to PMA (50 µg/ml) and ionomycin (1 µg/ml) (see figure "Patterns of Th1/Th2 cytokine induction". The relative amount of each of the 8 cytokines was also profiled using the Multi-Analyte ELISArray Kit.
Large amounts of IL-2 and IFNγ, and to a lesser extent TNFα, are induced throughout, and apparently beyond, the time course tested. Only more moderate amounts of IL4 and IL5 are induced, reaching a maximum at approximately 24 hours of stimulation. The even lower levels of IL10 and IL13 that are secreted also reach a maximum at 24 hours and appear to decrease again at later times. The amount of IL12 is not detectable after 48 hours of constant stimulation.
Multi-Analyte ELISArray Kits profile 12 cytokines in 6 cell supernatant (media), serum, or plasma samples using one simple protocol and one standardized development time (see figure "Multi-Analyte ELISArray plate layout"). Multi-Analyte ELISArray Kits provide researchers with a rapid assessment of the level of multiple proteins (cytokines and chemokines) in multiple samples.
Commercially available capture and detection antibodies are screened to achieve the highest possible sensitivity and linearity for each cytokine or chemokine assay. All assays are optimized under uniform conditions to allow simultaneous detection without compromising performance (see figure "Optimized ELISA conditions enable the same development or incubation time").
Multi-Analyte ELISArray Kits use a standard sandwich ELISA protocol (see flowchart "ELISArray protocol"). There is no requirement for special equipment; kits are compatible with a standard ELISA plate reader.
Multi-Analyte ELISArray Kits are highly suited for research into autoimmune and immune disorders, cancer, immunology, infectious diseases, and many other areas of biological and medical research. They can be used in any application typically analyzed using individual ELISA kits.
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