miScript miRNA Inhibitors

For studies on miRNA function and gene regulation using miRNA inhibition

  • Ready-to-transfect inhibitors bind and inhibit miRNAs
  • Available for all human, mouse, and rat miRNAs in miRBase
  • Highly flexible formats, scales, and grades
  • Custom design available for miRNAs not listed in miRBase

miScript miRNA Inhibitors are chemically synthesized, single-stranded, modified RNAs which specifically inhibit endogenous miRNA function after transfection into cells. miScript miRNA Inhibitors are available at cell-culture grade (>90% purity) or animal grade (HPLC purified). miScript miRNA Inhibitors are also available phosphorothioate modified at the 20 nmol scale. For flexible screening, miScript miRNA Inhibitor Plates enable researchers to order any predesigned or custom-designed miScript miRNA Inhibitors in their choice of layout in 96-well or 384-well plates. miScript miRNA Mimics can be used for similar experiments.

 

Please beware that miScript miRNA Inhibitors will be discontinued on July 1, 2019. Products will still be available for reordering, but the product webpage will be removed. We strongly recommend miRCURY LNA miRNA Inhibitors which have superior performance.

 

Search in GeneGlobe
You can search for the following terms:
  • Entrez Gene IDs (e.g., 835)
  • RefSeq IDs (e.g., NM_032983, NP_116765)
  • Gene symbols (e.g., CASP2)
  • Cat. no. (e.g., SI00299551, QT01342509)
  • Sanger ID or Accession (e.g., hsa-let-7b, MI0000063)
  • CpG loci identification numbers (CG#) (e.g., CG17753661)

Do not enter species information in the Search box. Use the drop-down list to select your species of interest.
When searching for miRNAs, do not omit the hyphens. Use hyphens or spaces (e.g., search for hsa-let-7b or hsa let 7b. Do not search for hsalet7b).

Advanced search settings



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产品 货号 目录价:
miScript miRNA Inhibitor
1 nmol (cell-culture grade), 5 nmol (cell-culture grade), or 20 nmol (animal grade, option of phosphorothioate modification) miScript miRNA Inhibitor, provided in tubes
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Details

Can’t order online or need plate formats? Order Forms and Plate Orders

miScript miRNA Inhibitors适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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miRNA Inhibitor在96个小时后的效能。|miScript miRNA Inhibitor可抵消miRNA诱导的基因沉默。|转染miRNA抑制剂会降低内源性miRNA的水平。|
使用HiPerFect Transfection Reagent将miR-15a Inhibitor、miR-16 Inhibitor、miR-21 Inhibitor、miR-25 Inhibitor或含有错义序列的Inhibitor(阴性对照)转染至HeLa S3细胞(6 x 104个细胞/孔)。在指定的时间点,转染带有适当的miRNA结合位点的荧光素酶报告基因重组体。使用不受任何miRNA调控的报告基因重组体作为阳性对照。24小时后,检测荧光素酶活性。|HeLa S3细胞高水平表达miR-16,但不表达miR-1(参见参考文献1)。在使用HiPerFect Transfection Reagent进行的共转染实验中,采用3' UTR带有miR-16结合位点的荧光素酶报告基因重组体及miR-16 Inhibitor共转染HeLa S3细胞(6 x 104个细胞/孔)。实验使用不同浓度的miR-16 Inhibitor,以评估观察抑制效应所需的最佳抑制剂浓度。另外单独转染miR-1 Inhibitor至细胞,作为对照。转染不带miRNA结合位点的荧光素酶重组体(WT),作为阳性对照。转染Inhibitor后,荧光素酶表达增加,说明miR-16 Inhibitor可阻止内源性miR-16下调报告基因的表达。
1. Lagos-Quintana, M. et al.(2001)Identification of novel genes coding for small expressed RNAs. Science 294, 853.
|使用HiPerFect Transfection Reagent将miScript miR-16 Inhibitor或阴性对照转染至HeLa S3细胞(6 x 104个细胞/孔)。使用miScript System,利用real-time PCR对内源性miR-16的水平进行定量。使用U6B作为对照RNA,用于数据标准化,miR-16的表达水平以相对于未转染细胞中的miR-16水平的2-ΔΔCT值表示。[A] 转染miScript miR-16 Inhibitor可显著降低检测到的内源性miR-16的水平。[B] 扩增曲线说明,转染miR-16 Inhibitor后,检测到的miR-16水平显著降低。解离曲线分析(插图)反映了使用miScript System进行real-time PCR检测的特异性。|
性能
miScript miRNA Inhibitors have been designed and modified to ensure efficient inhibition of endogenous miRNA function (see figure miScript miRNA Inhibitor counteracts miRNA-induced silencing). Real-time PCR analysis detected a significant decrease in miRNA level after transfection of miScript miRNA Inhibitor (see figure "Transfection of miRNA inhibitor decreases endogenous miRNA level"). Time-course experiments indicate that miScript miRNA Inhibitors are effective for at least 96 hours following transfection (see figure "miRNA inhibitor effective after 96 hours").
原理

Transfection of inhibitors, followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs.

Increased gene expression after transfection of inhibitor provides evidence that the miRNA under study is involved in regulation of that gene. Similar studies can be performed using miScript miRNA Mimics. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.

操作流程

Highly flexible solutions offered by miScript miRNA Inhibitors are available for every human, mouse, rat, and virus miRNA in the current version of the microRNA database, miRBase. QIAGEN's GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible. If the miRNA of interest is not available in miRBase, an inhibitor for your human, mouse, rat, or virus miRNA can be custom synthesized by visiting QIAGEN's custom miScript product design page.

Flexible formats enable either low-throughput analysis using small numbers of inhibitors or high-throughput screening using hundreds of inhibitors. miScript miRNA Inhibitors are available at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Inhibitors can be ordered at the 20 nmol scale (provided in tubes). miScript miRNA Inhibitors are also available phosphorothioate modified at the 20 nmol scale for in vivo applications. Phosphorothioate modification protects the inhibitor from exonuclease activity, increasesing the stability of the molecule. miScript miRNA Inhibitors can easily be ordered at GeneGlobe. For medium- to high-throughput applications, 96-well plates, or 384-well plates are available to suit your experimental requirements.

应用
miRNA research
Gene regulation research
特点
参数
设计 Predesigned and custom design avaliable
规格 Single tubes, 96-well & 384 well plates
修饰 Yes, optional with 20 nmol: phosphorothiate modified for in vivo studies
规模或产量 1 nmol, 5 nmol, 20 nmol
提供的序列 No
物种 Human, mouse, rat

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图片
miScript miRNA Inhibitor effective after 96 hours
miRNA Inhibitor在96个小时后的效能。
使用HiPerFect Transfection Reagent将miR-15a Inhibitor、miR-16 Inhibitor、miR-21 Inhibitor、miR-25 Inhibitor或含有错义序列的Inhibitor(阴性对照)转染至HeLa S3细胞(6 x 104个细胞/孔)。在指定的时间点,转染带有适当的miRNA结合位点的荧光素酶报告基因重组体。使用不受任何miRNA调控的报告基因重组体作为阳性对照。24小时后,检测荧光素酶活性。
miScript miRNA Inhibitor Counteracts miRNA-Induced Silencing
miScript miRNA Inhibitor可抵消miRNA诱导的基因沉默。
HeLa S3细胞高水平表达miR-16,但不表达miR-1(参见参考文献1)。在使用HiPerFect Transfection Reagent进行的共转染实验中,采用3' UTR带有miR-16结合位点的荧光素酶报告基因重组体及miR-16 Inhibitor共转染HeLa S3细胞(6 x 104个细胞/孔)。实验使用不同浓度的miR-16 Inhibitor,以评估观察抑制效应所需的最佳抑制剂浓度。另外单独转染miR-1 Inhibitor至细胞,作为对照。转染不带miRNA结合位点的荧光素酶重组体(WT),作为阳性对照。转染Inhibitor后,荧光素酶表达增加,说明miR-16 Inhibitor可阻止内源性miR-16下调报告基因的表达。
1. Lagos-Quintana, M. et al.(2001)Identification of novel genes coding for small expressed RNAs. Science 294, 853.
Transfection of miRNA Inhibitor Decreases Endogenous miRNA Level
转染miRNA抑制剂会降低内源性miRNA的水平。
使用HiPerFect Transfection Reagent将miScript miR-16 Inhibitor或阴性对照转染至HeLa S3细胞(6 x 104个细胞/孔)。使用miScript System,利用real-time PCR对内源性miR-16的水平进行定量。使用U6B作为对照RNA,用于数据标准化,miR-16的表达水平以相对于未转染细胞中的miR-16水平的2-ΔΔCT值表示。[A] 转染miScript miR-16 Inhibitor可显著降低检测到的内源性miR-16的水平。[B] 扩增曲线说明,转染miR-16 Inhibitor后,检测到的miR-16水平显著降低。解离曲线分析(插图)反映了使用miScript System进行real-time PCR检测的特异性。