Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID - 3732

With the Investigator Quantiplex Pro Kit, custom dye calibration for FAM, JOE, ATTO 550, ROX, and ATTO 647N is strongly recommended when using the Applied Biosystems 7500 Real-Time PCR System. To achieve optimal performance, please calibrate with the Investigator Quantiplex Pro Calibration Kit (cat. no. 387416). However, standard dye calibrations for FAM, JOE, TAMRA, ROX, and Cy5 might be used. In this case, use TAMRA for the Degradation target and Cy5 for the Male target.