Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125

Yes, Dithiothreitol (DTT) can be used in Buffer RLT Plus. The concentration should be 20ul of 2M DTT per 1mL of Buffer RLT Plus. The stock solution of 2 M DTT in water should be prepared fresh, or frozen in single-use aliquots. Buffer RLT Plus containing DTT can be stored at room temperature for up to 1 month.