Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?

Yes, Dithiothreitol (DTT) can be used in Buffer RLT Plus. The concentration should be 20ul of 2M DTT per 1mL of Buffer RLT Plus. The stock solution of 2 M DTT in water should be prepared fresh, or frozen in single-use aliquots. Buffer RLT Plus containing DTT can be stored at room temperature for up to 1 month.