QIAamp DSP DNA FFPE Tissue Kit
For purification of DNA from formalin-fixed paraffin-embedded tissues
The QIAamp DSP FFPE Tissue Kit provides silica-based DNA purification from formalin-fixed paraffin-embedded tissue. The QIAamp DSP DNA FFPE Tissue Kit is designed for labs that process FFPE tissues for in vitro diagnostic use.
The QIAamp DSP DNA FFPE Tissue Kit uses QIAamp MinElute spin columns for purification of high-quality DNA from FFPE tissue. Genomic DNA is efficiently purified from FFPE tissue sections without overnight incubation. The procedure partially removes formalin crosslinking of released DNA, for improved yield and DNA performance in downstream applications.
The QIAamp DSP DNA FFPE Tissue Kit uses well-established QIAamp MinElute technology for purification of genomic DNA from FFPE tissue. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 200 µl.
Specially optimized lysis conditions allow genomic DNA to be efficiently purified from FFPE tissue sections without the need for overnight incubation. Incubation at an elevated temperature after proteinase K digestion partially removes formalin crosslinking of the released DNA, improving yield as well as DNA performance in downstream assays. Note that DNA isolated from FFPE samples is usually of lower molecular weight than DNA from fresh or frozen samples. The degree of fragmentation depends on the type and age of the sample and the conditions used for fixation.
The QIAamp DSP DNA FFPE Tissue Kit uses special lysis conditions to release DNA from tissue sections and to overcome inhibitory effects cause by formalin cross-linking of nucleic acids. The procedure consists of 6 steps — remove paraffin, lyse, heat, bind, wash, and elute (see "Procedure"). Paraffin is dissolved in xylene and removed. The sample is lysed under denaturing conditions, with a short proteinase K digestion. Incubation at 90°C reverses formalin cross-linking. DNA binds to the membrane and contaminants are washed away. DNA is eluted in Buffer ATE and is immediately ready for use in amplification reactions or for storage at –20°C. The simple QIAamp DSP DNA FFPE Tissue Kit procedure is highly suited for simultaneous processing of multiple samples.
The QIAamp DSP DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed paraffin-embedded tissue.
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