[A] The proprietary PCR buffer contains Q-Bond, a molecule that facilitates the reduction of the annealing step to 5 seconds. Q-Bond increases the affinity of Taq DNA polymerases for short single-stranded DNA fragments, reducing the time needed for successful primer annealing. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing the duration of the annealing step.
[A] NH4+ ions prevent nonspecific primers from annealing to the template. Synthetic Factor MP, an innovative PCR additive, increases the local concentration of primers at the template. [B] Together with K+ and other cations, synthetic Factor MP stabilizes specifically bound primers, allowing efficient primer extension by DNA Polymerase.
The QuantiNova DNA Polymerase is kept in an inactive state by the QuantiNova Antibody and QuantiNova Guard until the initial heat activation step.
The master mix contains an inert blue dye. Combined with QuantiNova Yellow Template Dilution Buffer, the resulting solution turns green, indicating that the reaction was set up correctly.