E. coli DH5a strains carrying pCMVβ were grown in 5 ml LB medium. Plasmid DNA was isolated manually using the QIAvac 96 and either the QIAprep 96 Plus Miniprep Kit or the QIAprep 96 Turbo Miniprep Kit or automated on the BioRobot Universal System using either the QIAprep 96 Plus BioRobot Kit or the QIAprep 96 Turbo BioRobot Kit. DNA was quantified using absorbance measurement and verified by agarose gel analysis. Higher yields were achieved with QIAprep 96 Plus Miniprep and BioRobot Kits.
E. coli DH5a strains carrying pCMVβ were grown in LB medium. Preps were performed according to the manufacturer's instructions. DNA was quantified using absorbance measurement and verified by agarose gel analysis. The QIAprep 96 Plus Miniprep Kit provided the highest yields compared to the other kits tested.
pCMVβ DNA was prepared using the QIAprep 96 Plus Miniprep Kit and kits from other suppliers. For agarose gel analysis, 150 ng (as determined by absorbance measurement) of each sample was used. With the QIAprep 96 Plus Miniprep Kit, a high percentage of supercoiled plasmid DNA (lower band) was observed and absorbance measurements and agarose gel analysis were comparable, indicating superior plasmid DNA quality. M: GelPilot 1 kb Plus Ladder (the most intense fragment corresponds to 100 ng).