The DNeasy PowerPlant Pro Kit isolates high-quality genomic DNA from plant samples. Using a 1% TAE agarose gel, DNA was isolated from grass (2 µl), strawberry leaf (15 µl), grape leaf (15 µl) and pine needles (1 µl).
Using the DNeasy PowerPlant Pro Kit and plant DNA isolation kits from three other suppliers, DNA was isolated from cotton seeds. Isolations were performed per each manufacturer’s protocols. A 1% TAE agarose gel was used to examine a 2% elution of DNA volume.
Using RuBisCo primers run through qPCR on 1 ng of DNA from pine needles (A) and strawberry leaf (B), both diluted 1:10 and undiluted DNA samples fell within the standard curve (purple lines). The difference between the diluted and undiluted samples was approximately 3 cycles indicating that the samples were freed of PCR inhibitors.