Total RNA and genomic DNA were purified from 10 mg samples of rat liver using AllPrep technology (the AllPrep DNA/RNA Mini Kit). [A] Purified RNA samples were analyzed on a 1.2% formaldehyde agarose gel. [B] Purified DNA samples were analyzed on a 0.8% agarose gel. M: markers.
Genomic DNA and total RNA were purified from 10-fold serial dilutions of rat kidney homogenate using the AllPrep DNA/RNA Micro Kit; at each dilution, DNA was purified from duplicates, and RNA was purified from 4 replicates. [A]Real-time PCR analysis of the Pgk1 gene and [B] real-time RT-PCR analysis of the Jun transcript showed reliable quantification over a wide dynamic range, from 5 mg down to 50 ng tissue.