Metagenomics

Detecting microbial metagenomes

Metagenomic technologies enable the study of microbial genetic material in human biomedical sample types such as stool, nasal, oral, urogenital, skin and bronchoalveolar lavage samples. In addition, environmental samples such as soil, water, air and biofilms can be also be analyzed. Metagenomic data can not only be used to examine healthy microbiomes and shed light on causes, effects, and future therapies for a variety of diseases, but it can also be useful to help understand the environment's microbial biodiversity. QIAGEN provides next-generation sequencing technologies for metagenomics, as well as qPCR assays and arrays for verification of sequencing results and screening for specific bacterial species, virulence factor genes, and antibiotic resistance genes.

Sample collection

DNA purification

Sample enrichment

Library preparation

Verification by qPCR

Step 1: Sample collection

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	RNAlater RNA Stabilization Reagent For immediate stabilization of RNA in tissues Show details	See products
	LifeGuard Soil Preservation Solution For the ambient temperature stabilization of microbial RNA in the soil Show details	See products
	RNeasy Protect Saliva Mini Kit For stabilization and purification of total RNA from human saliva Show details	See products

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Step 2: DNA purification

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	Pathogen Lysis Tubes For mechanical disruption of bacteria and fungi, including yeast Show details	See products
	QIAamp DNA Stool Mini Kit For isolation of DNA from stool Show details	See products
	QIAamp UCP Pathogen Mini Kit For microbial DNA purification from whole blood, swabs, cultures, and body fluids Show details	See products
	QIAamp Fast DNA Stool Mini Kit For isolation of gDNA from stool samples Show details	See products
	QIAamp DNA Microbiome Kit For isolation of bacterial microbiome DNA from mixed samples Show details	See products
	QIAamp PowerFecal DNA Kit For the isolation of DNA from stool, gut material and biosolids Show details	See products
	RNeasy PowerMicrobiome Kit For the isolation of total RNA from stool and gut material Show details	See products
	MagAttract PowerMicrobiome DNA/RNA Kit For the automated, hands-free isolation of nucleic acids from stool and gut material Show details	See products
	DNeasy PowerSoil Kit For the isolation of microbial genomic DNA from all soil types Show details	See products
	DNeasy PowerLyzer PowerSoil Kit For the bead-based isolation of DNA from tough soil microbes Show details	See products
	DNeasy PowerMax Soil Kit For the isolation of microbial DNA from large quantities of soil with low microbial load Show details	See products
	DNeasy PowerSoil HTP 96 Kit For the isolation of DNA from up to 384 soil samples in less than one day Show details	See products
	MagAttract PowerSoil DNA Kit For the automated, hands-free isolation of DNA from soil Show details	See products
	MagAttract PowerWater DNA/RNA Kit For the automated isolation of nucleic acids from filtered air and water samples Show details	See products
	RNeasy PowerSoil Total RNA Kit For the isolation of high quality total RNA from all soil types Show details	See products
	RNeasy PowerSoil DNA Elution Kit For co-isolation of DNA and RNA from soil using the Rneasy PowerSoil Total RNA Kit Show details	See products
	RNeasy PowerWater Kit For the isolation of total RNA from filtered water samples Show details	See products
	RNeasy PowerBiofilm Kit For the isolation of high quality total RNA from biofilm samples Show details	See products
	DNeasy PowerWater Kit For the isolation of genomic DNA from filtered water samples, including turbid water Show details	See products
	DNeasy PowerWater Sterivex Kit For the isolation of genomic DNA from all filtered water sample types collected with Sterivex filter units Show details	See products
	AllPrep PowerViral DNA/RNA Kit For isolating viral or bacterial total nucleic acids from waste water and stool samples Show details	See products
	QIAamp BiOstic Bacteremia DNA Kit For the isolation of bacterial DNA from cultured blood, fecal and cervical swabs Show details	See products
	DNeasy PowerBiofilm Kit For the isolation of high-quality, pure DNA from biofilm samples Show details	See products

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Step 3: Sample enrichment

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	REPLI-g Single Cell Kit For highly uniform whole genome amplification (WGA) from single cells or limited sample material Show details		See products

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Step 4: Library preparation

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	GeneRead DNA Library l Core Kit For fast and efficient preparation of DNA libraries for use in NGS applications Show details	See products
	QIAseq cfDNA All-in-One Kits For conversion of cfDNA from plasma to NGS library for any liquid biopsy samples Show details	See products
	QIAseq FX Single Cell DNA Library Kit Single-cell whole genome libraries with comprehensive coverage and high sequence fidelity Show details	See products
	QIAseq FX Single Cell RNA Library Kit Single cell RNA-seq libraries that provide a deeper view of the transcriptome Show details	See products
	GeneRead DNA I Amp Kit For fast and efficient preparation of DNA libraries for use in NGS applications Show details	See products
	QIAseq FX DNA Library Kit High-quality whole genome libraries – no shearing required! Show details	See products
	GeneRead DNA L Core Kit For fast and efficient preparation of DNA libraries for use in NGS applications Show details	See products
	QIAseq 1-Step Amplicon Library Kit For fast and efficient preparation of DNA libraries for use in NGS applications Show details	See products
	GeneRead DNA L Amp Kit For fast and efficient preparation of DNA libraries for use in NGS applications Show details	See products
	QIAseq Ultralow Input Library Kit For preparation of DNA libraries for next-generation sequencing (NGS) applications that use Illumina® instruments Show details	See products
	QIAseq miRNA Library Kit Gel-free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next-generation sequencing Show details	See products
	GeneRead Library Quant System For qPCR-enabled quantification of NGS libraries Show details	See products

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Step 5: Verification by qPCR

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	Microbial DNA qPCR Arrays For real-time PCR-based, application-specific microbial identification or profiling Show details	See products
	Microbial DNA qPCR Assay Kits For real-time PCR-based microbial detection; includes one assay and everything required for a successful qPCR run for 20 reactions Show details	See products
	Microbial DNA qPCR Assays For real-time PCR-based microbial detection; includes one assay with Microbial qPCR Mastermix for 100 reactions Show details	See products
	Microbial DNA qPCR Multi-Assay Kits For real-time PCR-based, application-specific microbial identification or profiling; includes 3–6 assay tubes in one box for 20 reactions Show details	See products
	Custom Microbial DNA qPCR Arrays For real-time PCR-based, customized microbial identification or profiling Show details	See products

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