RNeasy 96 Universal Tissue 8000 Kit
For 96-well purification of total RNA from any tissue on the BioRobot Universal System
The RNeasy 96 Universal Tissue 8000 Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Automated purification is performed using the BioRobot Universal System, which processes up to 96 or 192 samples per run followed by real-time RT-PCR setup. Manual purification is carried out using the related RNeasy 96 Universal Tissue Kit. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAlater RNA Stabilization Reagent.
Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types (see table).
High-quality RNA can be isolated from tissues preserved either by freezing or by stabilization with RNAlater at room temperature (see figure "High-quality total RNA from rat liver and muscle tissue"). Intact RNA purified from difficult-to-lyse fibrous and fatty tissues is suitable for downstream applications (see figures "High-quality RNA from skin / adipose / liver / intestine tissue" and "Real-time analysis of high-quality RNA from rat brain").
The RNeasy 96 Universal Tissue 8000 Kit represents an advanced technology for high-throughput, automated RNA preparation, enabling purification of RNA from any animal or human tissue sample with the RNeasy 96 Universal Tissue procedure. Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types. The RNeasy procedure enriches for RNA >200 bases long, providing a yield of total RNA that does not include 5.8S rRNA, tRNA, and other low-molecular weight RNAs, which make up 15–20% of total cellular RNA.
The RNeasy 96 Universal Tissue 8000 Kit integrates efficient phenol/guanidine-based lysis and silica-membrane purification with the speed of vacuum processing. Following homogenization and phase separation, the BioRobot 8000 workstation or BioRobot Universal System provides walkaway automation of the procedure, for total RNA purification from all types of animal tissue (see flowchart "RNeasy 96 Universal Tissue 8000 procedure").
Tissue is first efficiently lysed using QIAzol Lysis Reagent and the TissueLyser II. This provides rapid and parallel disruption of tissues and inactivation of RNases to ensure purification of intact RNA. After phase separation by centrifugation, the BioRobot workstation removes the aqueous phase and adds ethanol to provide appropriate binding conditions. The workstation then applies the sample to the wells of the RNeasy 96 Universal Tissue plate, where total RNA binds and contaminants are efficiently washed away. High-performance RNA is then eluted in a small volume of water, ready for use in any downstream application.
The combination of QIAzol and RNeasy technologies results in highly pure RNA without phenol carryover. RNA purified using the RNeasy 96 Universal Tissue 8000 Kit is suitable for all downstream applications, including real-time RT-PCR.
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