Cat. No. / ID: 965662
The QIAamp Virus BioRobot 9604 Kit provides automated viral nucleic acid purification on the BioRobot 9604 using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours for up to 96 samples, including bar code reading and complete process documentation, with no hands-on time during the run.
QIAamp 96 plates provide well-to-well uniformity in RNA and DNA recovery for reliable results in downstream applications. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.
No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in efficient wash steps, leaving pure nucleic acids to be eluted in a buffer provided with the kit.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acid is then eluted in a low-salt buffer using the BioRobot 9604 (see flowchart " Protocol").
Highly pure, viral RNA and DNA can be isolated from 96 cell-free fluid samples in less than 2.5 hours. This kit can be used to isolate nucleic acids from human and animal viruses. QIAamp sample preparation technology is fully licensed.
The QIAamp Virus BioRobot 9604 Kit provides proven QIAamp technology in a convenient 96-well format for automated high-throughput purification of viral nucleic acids on the BioRobot 9604. The kit provides purification of nucleic acids from 200 µl or less of samples, including:
|For automated processing||BioRobot 9604 Workstation|
|Main sample type||Serum, plasma|
|Elution volume||50 µl|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||Viral DNA, viral RNA|
|Sample amount||<200 µl|
|Time per run or per prep||<2.5 hours|
QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.
QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.
For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.
Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.
Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).
Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.