For the bead-based isolation of DNA from tough soil microbes
Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer
Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid
Ready-to-use, high-quality DNA for downstream applications
Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minutes
Using novel Inhibitor Removal Technology, extract microbial DNA from all soil types with the DNeasy PowerLyzer PowerSoil Kit. Successful qPCR amplification is possible with isolated DNA of the highest level of purity. Processing a wide variety of environmental samples, especially those with a high humic acid content can be done effectively and efficiently with this kit.
The bead tubes contained in this kit are optimized for bead based homogenizers, particularly the PowerLyzer 24 Homogenizer, also available through QIAGEN. Pure microbial genomic DNA can be easily purified from compost, sediment, manure and other environmental samples and successfully amplified by qPCR. A variety of organisms have been detected from samples purified by the DNeasy PowerLyzer PowerSoil Kit, including both Gram (+/-) bacteria (e.g., Bacillus subtilis, Bacillus anthracis), fungi (e.g., yeasts, molds), algae and actinomycetes (e.g., Streptomyces) and nematodes. Genetic material processed is ready for a variety of downstream applications including next-generation sequencing.
Isolation of DNA using the DNeasy PowerLyzer PowerSoil Kit can be automated on the QIAcube Connect.
DNeasy PowerLyzer PowerSoil Kit was formerly sold by MO BIO as PowerLyzer PowerSoil DNA Kit.
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 96 adapters with different barcodes (pierceable foil seal, allowing usage of defined parts of plate)