QIAamp PowerFecal Pro DNA Kit

For the isolation of microbial DNA from stool and gut samples

Features

  • Efficient lysis of bacteria and fungi from stool and gut samples
  • Up to 20-fold more DNA compared to alternative methods
  • Yield higher alpha diversity in sequencing compared to other methods
  • Recovery of inhibitor-free DNA for direct use in next-generation sequencing applications
  • Automate workflows on the QIAcube Connect
undefined

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

QIAamp PowerFecal Pro DNA Kit

Cat. No. / ID: 51804

For the isolation of microbial DNA from stool and gut samples
NOK 4,120.00
Preparations
50
2
Add to cart
The QIAamp PowerFecal Pro DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

 

 

Extracting microbial DNA from stool and gut samples can be challenging. Researchers will isolate higher yields of pure microbial DNA from stool and gut samples more efficiently with our new QIAamp PowerFecal Pro DNA Kit.

 

The QIAamp Power Fecal Pro DNA Kit builds and improves on our original PowerFecal technology utilizing a novel bead tube and optimized chemistry for more efficient lysis of bacteria and fungi. Inhibitor removal has been streamlined, allowing researchers to eliminate challenging inhibitors from stool and gut samples faster. This results in total DNA that reveals higher alpha diversity through observed operational taxonomic units (OTUs) in downstream testing.

 

Isolation of DNA using the QIAamp PowerFecal Pro DNA Kit can be automated on the QIAcube Connect.

Want to try this solution for the first time? Request a quote for a trial kit.

Performance

Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool and gut samples compared with alternative methods (See: 'Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.').
Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool and fungal samples (See: 'The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.').

Principle

The streamlined Inhibitor Removal Technology® (IRT) used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit and other commercially available kits (See: ‘Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).

Procedure

Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid and thorough
homogenization occurs using mechanical and chemical methods. Once cells are lysed, our patented IRT is used to remove
inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted,
ready for downstream applications.

Supporting data and figures

Resources

Webinars (1)
This webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples.
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699