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MagAttract PowerSoil DNA Kit

For the automated, hands-free isolation of DNA from soil
  • Optimized for hands-free, automated processing
  • High-quality DNA isolation from tough soil, stool and other environmental samples
  • Inhibitor Removal Technology eliminates PCR-inhibiting compounds
  • Increased DNA yield with ClearMag technology that removes organic inhibitors
Isolate microbial DNA from soil and environmental samples with the MagAttract PowerSoil DNA Kit. Optimized for automated systems, the MagAttract PowerSoil DNA Kit processes up to 0.25 g of soil, sediment, compost or stool. The combination of Inhibitor Removal Technology and ClearMag magnetic particle technology enables the purification of DNA without organic inhibitors, providing high-quality, pure DNA for downstream applications.

Protocols are available for the Eppendorf epMotion and Thermo Scientific KingFisher, but this kit can also be used with a host of automated processing and liquid handling systems.

The MagAttract PowerSoil DNA Kit was formerly sold by MO BIO as PowerMag Soil DNA Isolation Kit.
Cat No./ID: 27000-4-KF
MagAttract PowerSoil DNA KF Kit
€2,178.00
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For the automated, hands-free isolation of DNA from soil
Cat No./ID: 27000-4-KF-3
ClearMag Binding Solution (200 ml)
€201.00
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MagAttract PowerSoil DNA KF Kit Components (Optimized for KingFisher).
Cat No./ID: 27100-4-EP
MagAttract PowerSoil DNA EP Kit (384)
€2,178.00
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For the hands-free isolation of DNA from soil using automated processing and liquid handling systems
Cat No./ID: 27100-4-EP-BP
MAPS PowerBead DNA Plates, Garnet (4)
€346.00
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MagAttract PowerSoil DNA Isolation Kit Components (For Automated Liquid Handling Systems)
The MagAttract PowerSoil DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1.
Inhibitor Removal Technology (IRT) is essential for removal of PCR inhibitors. DNA was isolated from organic garden soil samples using the MagAttract PowerSoil DNA Kit with and without the IRT step and purified using the KingFisher Duo. QPCR was performed as described in Figure 2. Samples processed without IRT failed to amplify, while samples with IRT were successfully amplified and fell within the standard curve (blue lines).
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Figure 2.
High quality, pure DNA isolated from a panel of six soil samples (0.25 g) using the MagAttract PowerSoil DNA Kit on the KingFisher Duo. High quality, high molecular weight DNA was observed in each soil sample with yields varying based on microbial load. No differences in yield or quality were observed between the replicate samples. 
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Figure 3.
QPCR was performed on 1 µl of DNA from organic garden soil (a), low biomass soil (b) and landscaping soil (c), all of which are known to be high in PCR inhibitors, using primers for Bacillus. Undiluted DNA and samples diluted 1:10 fell within the standard curve (grey lines), and the difference between the diluted and undiluted samples was approximately 3 cycles, indicating the DNA was free of inhibitors.
Performance



Features
Specifications
Bead size 0.7 mm garnet
Binding capacity 500 µg per 20 µl of beads
Format ClearMag Technology
Processing Bead beating
Sample size 0.25 g
Sample types Processed soil and stool
Storage temperature RNase A should be stored at 4°C The other kit reagents and components should be stored at room temperature (15-30°C)
Throughput 96-well plate

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