DNeasy Blood & Tissue Kits

For spin-column or 96-well purification of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses

  • Standardized method for a variety of sample types
  • High yields even from specialized samples
  • High-quality DNA
  • Optimized protocols for a range of starting materials
  • Spin-column and 96-well high-throughput formats

DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA purification in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible purification of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Purification of DNA using the DNeasy Blood & Tissue Kit can be automated on the QIAcube Connect.

产品 Product no. 货号 目录价:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
产品 货号 目录价:
DNeasy 96 Blood and Tissue Kit (4)
For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers
69581
$1,613.00
加入购物车
DNeasy 96 Blood and Tissue Kit (12)
For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers
69582
$4,293.00
加入购物车
DNeasy Blood and Tissue Kit (50)
50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
69504
$206.00
加入购物车
DNeasy Blood and Tissue Kit (250)
250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
69506
$884.00
加入购物车

DNeasy Blood & Tissue Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


  • Main Image Navi
DNA产量。|DNeasy Mini和96实验流程。|高效的16重PCR。|高品质DNA。|对马进行基因分型。|高通量纯化。|Real-time PCR。|对猪进行基因分型。|
使用DNeasy Blood & Tissue Kit从100 µl全血中纯化DNA。羊:从50 µl羊全血中纯化DNA。使用超过50 µl的羊血时,DNA产量没有明显增加。鸡:从5 µl鸡全血中纯化DNA。鸟类血液中含有核红细胞,因此其DNA产量比哺乳动物的高。||使用DNeasy Blood & Tissue Kit从人类K562细胞中纯化基因组DNA。使用QIAGEN Multiplex PCR Kit对图示量的DNA和16对引物进行多重PCR,反应平行进行两次。M: 100 bp ladder。|使用DNeasy Blood & Tissue Kit从图示的动物样本中纯化DNA,并进行琼脂糖凝胶电泳分析。对于每个样本,都对0.5 µg未经过酶处理的DN(左侧)、或1 µg经过EcoRI处理的DNA(右侧)进行了分析。M: lambda-HindIII。|从三批棕色马的鬃毛中纯化获得DNA,并使用MSH和ASIP PCR对毛色基因型进行分析。[A] MSH PCR产物用TaqI酶处理。马1和2确定为Ee杂合体,携带红色毛色的隐性等位基因e。[B]对ASIP PCR产物分析得出,马1和2为Aa杂合体,携带黑色毛色的隐性等位基因a。+:黑色马Ee aa阳性对照。阴性对照。M:标记物。|使用DNeasy 96 Blood & Tissue Kit从24个小鼠尾和24个大鼠尾样本中纯化DNA。在1%的琼脂糖凝胶上进行DNA电泳分析。M: lambda-HindIII。|Borrelia sp.是由蜱携带的病原体,可导致人类或其他哺乳动物患莱姆病。使用DNeasy Blood & Tissue Kit从蜱中纯化DNA。在LightCycler仪器上使用artus Borrelia LC PCR Kit,通过real-time PCR检测Borrelia DNA。阳性样本曲线的最低处对应每次PCR反应5个拷贝DNA。|使用DNeasy Blood & Tissue Kit从猪耳刻样本中纯化DNA,然后使用Biotype AG公司的Animaltype Pig PCR Amplification Kit和QIAGEN HotStarTaq DNA Polymerase进行扩增。电泳图显示:12个多态性四核苷酸微卫星和一个性别鉴定标记物(SBH23)的遗传指纹图谱。
|
性能

The efficient DNeasy Blood & Tissue procedure enables high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using DNeasy Blood & Tissue Kits" and figure "DNA yields"). Optimized protocols ensure high yields from nonstandard samples, such as animal hair (see figure "Genotyping of horses"), as well as cultured cells, fixed tissues, or Gram-positive and -negative bacteria. Specialized online protocols are available for DNA purification from yeast, insects, hair, and other sample types.

Typical yields from animal tissues using DNeasy Blood & Tissue Kits
SourceAmount DNA(µg)
Mammalian blood 100 µl 3–6
Bird blood 5 µl 9–40
HeLa cells 2 x 106 15–25
Liver 25 mg 10–30
Brain 25 mg 15–30
Kidney 25 mg 15–30
Spleen 10 mg 5–30
Mouse tail 1.2 cm (tip) 10–25
Rat tail 0.6 cm (tip) 20–40
Pig ear 25 mg 10–30
Horse hair 10 hairs 2–4
Fish fin 20 mg 10–20
Fish spawn (mackerel) 10 mg 5–10

DNeasy Blood & Tissue Kits simplify purification of DNA from a wide range of sample types, including animal species commonly encountered in life science, veterinary, and genotyping applications (see figure "High-quality DNA"). Purified DNA is free from PCR inhibitors, enabling sensitive detection in standard, multiplex (see figure "Efficient 16plex PCR"), and real-time PCR (see figure "Real-time PCR"). DNeasy Blood & Tissue Kits provide reliable results — from laboratory analysis of transgenic mice (see figure "High-throughput purification") to livestock breeding programs (see figure "Genotyping of pigs") and pedigree genotyping. Routine testing applications can be easily scaled up using the DNeasy 96 Blood & Tissue Kit (see figure "High-throughput purification").

原理

DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.

The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.

Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.

操作流程

Reliable silica-membrane technology, in convenient spin-column or 96-well formats, ensures fast and reproducible DNA purification, eliminating the need for organic extraction and alcohol precipitation (see flowchart "DNeasy Mini and 96 procedures"). Samples are first lysed using proteinase K. Buffering conditions are adjusted to provide optimal DNA-binding conditions and the lysate is loaded onto the DNeasy Mini spin column or the DNeasy 96 plate. During centrifugation, DNA is selectively bound to the DNeasy membrane as contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in two efficient wash steps and DNA is then eluted in water or buffer, ready for use.

DNeasy Mini spin columns are prepackaged in collection tubes and individually sealed, providing convenience and safety. The purification procedure using DNeasy Mini spin columns can be automated on the QIAcube. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System.

应用

DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:

Life science research
Livestock breeding
Pedigree genotyping
Veterinary pathogen research
Routine applied testing
特点
参数
应用 PCR, real-time PCR, genotyping
洗脱体积 100–200 µl
规格 96-well plate, spin column
主要样本类型 Blood, tissue
处理 Manual
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 DNA
样本量 100 µl/25 mg
技术 Silica technology
每次运行或制备时间 20 minutes – 1 hour
产量 6 µg/30 µg

您无权限查看此资源

常见问题
25
FAQ ID -12
浏览
FAQ ID -100
浏览
FAQ ID -129
浏览
FAQ ID -1253
浏览
FAQ ID -1254
浏览
FAQ ID -1255
浏览
FAQ ID -2953
浏览

浏览
FAQ ID -374
浏览
FAQ ID - 3517
浏览
FAQ ID -632
浏览
FAQ ID -728
浏览
FAQ ID -730
浏览
FAQ ID -904
浏览
FAQ ID -908
浏览
FAQ ID -909
浏览
FAQ ID -913
浏览
FAQ ID -917
浏览
FAQ ID -924
浏览
FAQ ID -929
浏览
FAQ ID -931
浏览
FAQ ID -934
浏览

浏览
FAQ ID - 3362
浏览
FAQ ID - 3447
浏览
补充实验方案
3
This protocol is designed for purification of DNA from up to 5 x 107 yeast cells.
显示更多
This protocol is designed for purification of DNA from up to 50 mg of insects, such as drosophila.
显示更多
This protocol is designed for purification of DNA from a 200 μl crude lysate.
显示更多
试剂盒操作手册
1
For purification of total DNA from animal blood, animal tissue, rodent tails, ear punches, cultured cells, fixed tissue, bacteria, insects

显示更多
参考文献
0
图片
Typical DNA Yields from Animal Blood Using DNeasy Blood & Tissue Kits
DNA产量。
使用DNeasy Blood & Tissue Kit从100 µl全血中纯化DNA。羊:从50 µl羊全血中纯化DNA。使用超过50 µl的羊血时,DNA产量没有明显增加。鸡:从5 µl鸡全血中纯化DNA。鸟类血液中含有核红细胞,因此其DNA产量比哺乳动物的高。
DNeasy Tissue and DNeasy 96 Tissue Procedure
DNeasy Mini和96实验流程。
Efficient 16plex PCR over a range of template amounts
高效的16重PCR。
使用DNeasy Blood & Tissue Kit从人类K562细胞中纯化基因组DNA。使用QIAGEN Multiplex PCR Kit对图示量的DNA和16对引物进行多重PCR,反应平行进行两次。M: 100 bp ladder。
High-Quality DNA from Various Animal Tissues
高品质DNA。
使用DNeasy Blood & Tissue Kit从图示的动物样本中纯化DNA,并进行琼脂糖凝胶电泳分析。对于每个样本,都对0.5 µg未经过酶处理的DN(左侧)、或1 µg经过EcoRI处理的DNA(右侧)进行了分析。M: lambda-HindIII。
Genotyping with DNA from Horse Hairs
对马进行基因分型。
从三批棕色马的鬃毛中纯化获得DNA,并使用MSH和ASIP PCR对毛色基因型进行分析。[A] MSH PCR产物用TaqI酶处理。马1和2确定为Ee杂合体,携带红色毛色的隐性等位基因e。[B]对ASIP PCR产物分析得出,马1和2为Aa杂合体,携带黑色毛色的隐性等位基因a。+:黑色马Ee aa阳性对照。阴性对照。M:标记物。
High-Throughput DNA Purification Using the DNeasy 96 Blood & Tissue Kit
高通量纯化。
使用DNeasy 96 Blood & Tissue Kit从24个小鼠尾和24个大鼠尾样本中纯化DNA。在1%的琼脂糖凝胶上进行DNA电泳分析。M: lambda-HindIII。
Variable elution volumes allow a range of yields and concentrations
Real-time PCR。
Borrelia sp.是由蜱携带的病原体,可导致人类或其他哺乳动物患莱姆病。使用DNeasy Blood & Tissue Kit从蜱中纯化DNA。在LightCycler仪器上使用artus Borrelia LC PCR Kit,通过real-time PCR检测Borrelia DNA。阳性样本曲线的最低处对应每次PCR反应5个拷贝DNA。
Genotyping of Pigs for Quality Management of Livestock
对猪进行基因分型。
使用DNeasy Blood & Tissue Kit从猪耳刻样本中纯化DNA,然后使用Biotype AG公司的Animaltype Pig PCR Amplification Kit和QIAGEN HotStarTaq DNA Polymerase进行扩增。电泳图显示:12个多态性四核苷酸微卫星和一个性别鉴定标记物(SBH23)的遗传指纹图谱。