EpiTect ChIP qPCR Arrays

EpiTect ChIP qPCR Arrays provide high sensitivity, specificity, and reproducibility using SYBR^® Green-based real-time PCR technology.

Reproducibility

The complete EpiTect ChIP qPCR Array system demonstrates a high degree of reproducibility across technical replicates, lots, and instruments. This consistency ensures reliable detection of differences in genomic DNA enrichment among biological samples. See figures "Consistent intra- and inter-plate performance" and "Consistent performance with different amounts of DNA, instruments, or handling conditions".

Specific and accurate ChIP-qPCR detection

One prerequisite for ChIP qPCR array technology is uniform and high PCR amplification efficiency, which allows a reciprocal comparison of ChIP enrichment among all genomic loci analyzed. The unique combination of a proprietary ChIP-PCR primer design algorithm, rigorous experimental verification of every EpiTect ChIP qPCR Assay, and high performance RT² SYBR^® Green Mastermix ensures the superior performance of EpiTect ChIP qPCR Arrays. See figure "Uniform amplification efficiency and specific PCR detection".

Regulating gene expression requires dynamic but regulated interactions between genomic DNA and proteins, such as transcription factors, coactivators, corepressors and modified histones. One important technique for studying the discovery of new and the timing and extent of known in vivo protein–DNA binding is chromatin immunoprecipitation (ChIP). Chemical crosslinking at the end of a biological experiment covalently captures and freezes the protein–DNA interactions. Sonication shears the chromatin into manageable sizes for the sensitive detection of specific genomic DNA sequences. Standard immunoprecipitation pulls down a target protein of interest and its bound DNA. Crosslink reversal and DNA purification followed by real-time PCR detection of specific sequences then quantifies the amount of protein-bound DNA.

EpiTect ChIP qPCR Arrays represent a focused panel of genomic regions relevant to a biological function or disease state. The target-sequence-specific assays in each well are designed with a proprietary algorithm developed to specifically to address the challenges presented by genomic DNA sequences. Each assay is also experimentally verified for the high amplification efficiency and specificity expected of optimal SYBR Green-based real-time PCR assays. The performance of the EpiTect ChIP qPCR Arrays is guaranteed when used with the appropriate RT² SYBR Green qPCR Mastermix.

EpiTect ChIP PCR Arrays are available in both 96- and 384-well plates and are used to monitor the binding of a target protein to the genomic loci of 84 different genes related to a disease state or pathway. Positive and negative controls for protein-specific immunoprecipitation are also included on each array, as well as controls for general PCR performance.

Available array formats

EpiTect ChIP qPCR Arrays are available in the following formats:

EpiTect ChIP qPCR Array Format A: 96-well plates containing dried qPCR primer assays, Optical Thin-Wall 8-Cap Strips

EpiTect ChIP qPCR Array Format C: 96-well plates containing dried qPCR primer assays, Optical Adhesive Film

EpiTect ChIP qPCR Array Format D: 96-well plates containing dried qPCR primer assays, Optical Thin-Wall 8-Cap Strips

EpiTect ChIP qPCR Array Format E: 384-well plate(s) containing dried qPCR primer assays, Optical Adhesive Film, 384EZLoad Covers

EpiTect ChIP qPCR Array Format F: 96-well plates containing dried qPCR primer assays, Optical Adhesive Film

EpiTect ChIP qPCR Array Format G: 384-well plate(s) containing dried qPCR primer assays, Optical Adhesive Film, 384EZLoad Covers

EpiTect ChIP qPCR Arrays can be used for research into cancer, immunology, stem cells, toxicology, and biomarker discovery and verification, and are also powerful tools for studying regulatory mechanisms behind the gene expression changes observed with RT² Profiler PCR Arrays and Assays. EpiTect ChIP qPCR Arrays provide a reliable tool for the analysis of a panel of ChIP-enriched genomic sequences associated with transcription factors and co-regulators, modified and unmodified histones, and other DNA-binding proteins.

EpiTect ChIP qPCR Arrays also streamline:

Identification of disease biomarkers

Characterization of signal transduction pathways

Examination of transcriptional regulation

Detection of epigenetic changes

Verification of ChIP-on-chip and ChIP-Seq data