pigtype PRRSV Ab and pigtype PRRSV Ab OF
For detection of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV)
pigtype PRRSV Ab is a highly specific and sensitive ELISA for detection of antibodies to PRRSV (EU and NA virus types) in serum and plasma samples from pigs. For reliable testing of oral fluid samples from pigs we provide the pigtype PRRSV Ab OF. Both kits are effective and suitable diagnostic tools to monitor the infection and vaccination status of pig herds over extended time periods and for controlling the serological status of purchased animals.
Assays for veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area.
Principle
PRRSV is an RNA virus that is a member of the family Arteriviridae, order Nidovirales. PRRS viruses are classified into the European (EU/I) and the North American (NA/II) genotypes. PRRS virus infections can cause respiratory disease in piglets and reproductive failure in pregnant sows.
The pigtype PRRSV Ab and the pigtype PRRSV Ab OF are indirect ELISAs for detection of these viruses. The microtiter test plate is coated with recombinant PRRSV antigens, specific for NA and EU virus types. During sample incubation, antibodies specific for PRRSV bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies specific for PRRSV are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620–650 nm) is optional. The OD value correlates to the concentration of antibodies specific for the NA/EU strains of PRRSV in the sample.
Applications
The pigtype PRRSV Ab and pigtype PRRSV Ab OF are highly sensitive and specific solutions for detection of antibodies to PRRSV (NA and EU virus types) in serum/plasma and oral fluid samples from pigs, respectively.
For further information and validation data on QIAGEN assays, contact QIAGEN Technical Service. Recommended for you
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