Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878

Yes, assays that make use of the biotin moiety for protein detection (e.g., streptavidin) are greatly facilitated when using the EasyXpress Site-Specific Biotin Kit for in vitro translation, because endogenous biotinylated proteins normally present in prokaryotic lysates have been removed.