Why is no signal detected in a lane?
FAQ -9015

First, check that the glass capillaries at the bottom of the cartridge are intact. You should see approximately 1 mm of glass capillary. Second, check that each tube has at least 10 µl sample volume.

Check the capillary is unblocked by performing the gel-droplet test as outlined in Appendix D in the QIAxcel DNA handbook.

Perform a detector test.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.