Glutathione HiCap Matrix shows a higher binding capacity compared to a resin from an alternative supplier. *Fast-flow resin.
Glutathione HiCap Matrix has a superior binding capacity compared to resins from other suppliers. Glutathione HiCap Matrix is a new and improved alternative to QIAGEN's Glutathione Superflow, and provides higher yields and a 50% increase in binding capacity. *Fast-flow resin; †High-performance resin; ‡Glutathione resin.
Yield of GST-tagged protein achieved using the Glutathione HiCap Cartridge (1 ml) depends on flow rates. Dynamic binding capacity is approximately 20 mg/ml; recommended flow rate for the 1 ml cartridge: 0.25–1 ml/min.
Purification of 19 mg of GST-tagged protein was performed using Glutahione HiCapMatix. M: Marker; CL: Cleared lysate; FT: Flow-through; W: Wash; E: Eluate. Analysis was performed by SDS-PAGE.
Purification of GST-tagged protein was performed with Glutathione HiCap Matrix and resins from various other suppliers. High purity was achieved using QIAGEN's new Glutathione HiCap Matrix. M: Marker; CL: Cleared lysate. Elution fractions are shown. Analysis was performed by SDS-PAGE. *Glutathione resin; †Fast-flow resin; ‡High-performance resin