Using the EZ1 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A260) with correction for background (A320). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.
RNA was purified from 2-3 replicate tissue samples from each of 4 types of rat tissue using the EZ1 RNA Tissue Mini procedure. Purified total RNA was examined by scanning spectrophotometry. [A] Kidney, 10 mg; [B]liver, 10 mg; [C] pancreas, 5 mg; [D]spleen, 5 mg.