miScript Microfluidics PreAMP Kit
For preamplification of cDNA prior to real-time PCR miRNA analysis using the Fluidigm BioMark System
The miScript Microfluidics PreAMP Kit performs preamplification to enable miRNA quantification on the Fluidigm BioMark System with a 96.96 or 48.48 Dynamic Array IFC. The miScript PCR System for microfluidics represents a breakthrough technology enabling accurate and comprehensive miRNA expression analysis on the Fluidigm BioMark System with as little as 10 ng total RNA. Highly multiplex, PCR-based preamplification of up to 400 miRNA-specific cDNA targets in one reaction results in a 1000–4000-fold amplification of each target. Preamplification dramatically reduces the sample requirements for analysis. A single 1 ng cDNA synthesis reaction can be used as template for up to 10 preamplification reactions.
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