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REPLI-g Single Cell DNA Library Kit

For DNA library construction from single cells for Illumina sequencing applications
  • MDA-based, PCR-free single cell library construction
  • Complete and accurate sequence coverage from a single cell
  • Fast time-to-result due to a streamlined protocol
  • High-quality libraries, ready for use on any Illumina NGS platform

Single cell genomic analysis enables researchers to gain novel insights across a diverse set of applications, including, developmental biology, tumor heterogeneity and disease pathogenesis and progression. Conducting single-cell genomic analysis using next-generation sequencing (NGS) methods has traditionally been challenging since the amount of genomic DNA present in a single cell is very limited. PCR-based whole genome amplification methods normally have high error rates, low coverage uniformity, extensive allelic drop-outs and limited amplification yields. The new REPLI-g Single Cell DNA Library Kit leverages QIAGEN's unique multiple displacement amplification (MDA) technology to overcome these challenges by preparing a sequencing library with high fidelity and minimal bias, while retaining the sample's genomic diversity.

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