For automated purification of viral nucleic acids or bacterial DNA from 1–96 samples
High reproducibility through standardized processing
Bar codes enable tracking of samples and reagents
Optimized protocols provide high nucleic acid yields
Novel, prefilled reagent cartridges for ease of use
QIAsymphony Virus/Bacteria Kits provide automated purification of viral nucleic acids and bacterial DNA from a broad range of sample types on the QIAsymphony SP. For example, nucleic acids can be purified from plasma, serum, CSF, respiratory samples, urine, urogenital swabs, and transport media. Kits are available in mini and midi format for sample volumes of 200 μl and up to 1000 μl. Optimized protocols provide high yields of pure nucleic acids, and flexible elution volumes allow nucleic acid concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories
QIAsymphony Virus/Bacteria Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Sensitive detection of Chlamydia trachomatis in urine.
DNA was purified from 800 μl urine with elution in 110 μl. Bacterial DNA was detected using a commercially available assay amplifying a specific sequence of the C. trachomatis genome on an ABI PRISM 7900HT SDS.
Efficient processing of magnetic particles.
The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.
Prefilled, sealed reagent cartridges.
Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use giving you complete cost control.
Performance
QIAsymphony Virus/Bacteria Kits enable robust and reproducible capture of nucleic acids. Purified viral and bacterial nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications. Efficient yields and flexible elution volumes (60 μl, 85 μl, or 110 μl) to suit the requirements of downstream applications ensure sensitive analytical detection (see figure "Sensitive detection of Chlamydia trachomatis in urine").
Principle
QIAsymphony Virus/Bacteria Kits combine the speed and efficiency of silica-based purification of viral and bacterial nucleic acids with the convenient handling of magnetic particles (see figure "Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
Procedure
The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
QIAsymphony Virus/Bacteria protocols
Protocol
Kit
Sample material
Processed volume
Cellfree 200
QIAsymphony Virus/Bacteria Mini Kit (192)
Plasma, serum, and CSF
200 μl
Cellfree 500
QIAsymphony Virus/Bacteria Midi Kit (96)
Plasma, serum, and CSF
500 μl
Cellfree 1000
QIAsymphony Virus/Bacteria Midi Kit (96)
Plasma, serum, and CSF
1000 μl
Complex 200
QIAsymphony Virus/Bacteria Mini Kit (192)
Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)
200 μl
Complex 400
QIAsymphony Virus/Bacteria Midi Kit (96)
Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)
400 μl
Complex 800
QIAsymphony Virus/Bacteria Midi Kit (96)
Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)
800 μl
Applications
QIAsymphony Virus/Bacteria Kits enable highly sensitive detection of a broad range of DNA and RNA viruses, as well as bacterial pathogens. Yields are linear, allowing accurate quantitative analysis for both low and high viral or bacterial titers. The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.
Nonsterile polypropylene tubes (0.85 ml maximum capacity, less than 0.7 ml storage capacity, 0.4 ml elution capacity); 2304 in racks of 96; includes cap strips
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)
