QIAamp UltraSens Virus Kit

For concentration and purification of viral RNA and DNA from serum and plasma
  • Rapid purification of high-quality, ready-to-use viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp UltraSens Virus Kit uses a novel technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. Starting with sample volumes of up to 1 ml, nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation.

产品 货号 目录价:
QIAamp UltraSens Virus Kit (50)
For 50 viral nucleic acid preps: 50 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
53704
询价
QIAamp UltraSens Virus Kit (250)
For 250 viral nucleic acid preps: 250 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
53706
询价

QIAamp UltraSens Virus Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


  • Main Image Navi
QIAamp UltraSens实验流程。|纯化获得的核酸在下游分析中有出色表现。|在RT-qPCR中的出色表现。|
|利用QIAamp UltraSens步骤从典型的病毒中纯化RNA,在LightCycler上进行一步法RT-PCR。geq/ml:每毫升中的基因组当量;:阴性对照;M:分子量标准。(数据由德国汉堡Artus GmbH的Thomas Laue博士提供。)|利用QIAamp UltraSens步骤从典型的病毒中纯化RNA,在LightCycler上进行定量real-time RT-PCR。geq/ml:每毫升中的基因组当量;:阴性对照;M:分子量标准。|
性能

The QIAamp UltraSens Virus Kit is suitable for purification of viral nucleic acids from a wide range of viral species. It can also be used for efficient isolation of extracellular genomic DNA from plasma and serum. The purified nucleic acids perform well in sensitive downstream applications (see figures "High performance in one-step PCR" and "High performance in RT-PCR").

The purified DNA and RNA can be used in a wide range of downstream applications, including:

PCR and quantitative real-time PCR
Infectious disease research
原理

No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit.

QIAamp technology yields viral RNA and DNA from cell-free body fluids that are ready to use in PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

操作流程

Nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation. This step allows nucleic acid purification from large sample volumes without the inconvenience of handling large volumes throughout the protocol. Viral nucleic acids are then purified using QIAamp silica-gel-membrane technology (see flowchart "Procedure"). Lysates are loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.

No ultracentrifugation or specialized laboratory equipment is required, and an internal control can be added at the start of the procedure, allowing full monitoring of the purification process.

应用

The QIAamp UltraSens Virus Kit uses new technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. The procedure provides increased sensitivity in viral-load monitoring and other applications where high viral nucleic acid recovery is essential. Samples of 1 ml plasma or serum can be prepared within 1 hour, with typical yields of >85% recovery of nucleic acids and 60 µl elution volume.

您无权限查看此资源

试剂盒操作手册
2

显示更多
For highly efficient purification of viral RNA and DNA from 1 ml plasma and serum samples
显示更多
安全数据表
1
Download Safety Data Sheets for QIAGEN product components.
浏览
图片
QIAamp UltraSens Procedure
QIAamp UltraSens实验流程。
High Performance of Purified Nucleic Acids in Downstream Analyses
纯化获得的核酸在下游分析中有出色表现。
利用QIAamp UltraSens步骤从典型的病毒中纯化RNA,在LightCycler上进行一步法RT-PCR。geq/ml:每毫升中的基因组当量;:阴性对照;M:分子量标准。(数据由德国汉堡Artus GmbH的Thomas Laue博士提供。)
High Performance of Purified Nucleic Acids in Downstream Analyses
在RT-qPCR中的出色表现。
利用QIAamp UltraSens步骤从典型的病毒中纯化RNA,在LightCycler上进行定量real-time RT-PCR。geq/ml:每毫升中的基因组当量;:阴性对照;M:分子量标准。