For protease digestion during DNA and RNA preparation
QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures as well as in a wide range of salt, denaturant, and detergent (see table "Protease activity in commonly used buffers"), pH, and temperature conditions. Both enzymes are quality-guaranteed by QIAGEN.
QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:
QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain and is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:
Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.
Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.
Note: QIAGEN Protease is not compatible with Buffer ATL in DNeasy Tissue, DNeasy 96 Tissue, and the QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl, or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.
Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.
QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.
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