For purification of genomic and mitochondrial DNA from small samples
- Rapid purification of high-quality DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.
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QIAamp DNA Micro Kit (50)
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)
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56304
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The QIAamp DNA Micro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp DNA Micro procedure.|Laser microdissection PCR.|Efficient purification of DNA from small sample sizes.|
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.|DNA was purified from rodent kidney laser microdissection (LMD) samples using the QIAamp DNA Micro Kit or using a direct lysis PCR protocol, as indicated. GAPDH PCR was performed on 1 and 10 µl of each eluate. M: Marker; +: positive control; –: negative control.|DNA was purified from blood samples (1 –64 µl) using the QIAamp DNA Micro Kit following the blood protocol. DNA was eluted in 20 µl Buffer AE and 2 µl of each eluate was amplified by real-time PCR. Efficient amplification was shown in all cases.|
Performance
The resulting purified DNA is free of proteins, nucleases, and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR"). DNA purified with the QIAamp DNA Micro Kit may also be used in short-tandem repeat (STR) genotyping, single-nucleotide pylomorphism (SNP) genotyping, or pharmacogenomic research.
Principle
The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. DNA binds specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.
QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.
Procedure
The QIAamp DNA Micro Kit simplifies isolation of DNA from small samples with a fast spin-column procedure (see flowchart " Procedure"). The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine, or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue, and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.
Applications
The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including:
- Small volumes of blood
- Blood cards
- Urine
- Small tissue samples, including laster microdissections
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Feature
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Specifications
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Applications
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Real-time PCR, STR analysis, LMD-PCR
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Elution volume
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20–100 µl
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Format
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Spin column
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Main sample type
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Whole blood
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Processing
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Manual (centrifugation or vacuum)
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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Genomic DNA, mitochondrial DNA
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Sample amount
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1–100 µl
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Technology
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Silica technology
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Time per run or per prep
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30 minutes
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Yield
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<3 µg
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FAQ ID -12
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FAQ ID -1188
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FAQ ID -1547
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I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
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FAQ ID -473
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FAQ ID -618
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FAQ ID -728
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FAQ ID -730
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FAQ ID -754
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FAQ ID -761
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FAQ ID -908
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FAQ ID -909
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FAQ ID -926
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FAQ ID -305
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For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA
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