QIAamp DNA Micro Kit

For purification of genomic and mitochondrial DNA from small samples

Rapid purification of high-quality DNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors

The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.

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Product		Cat. no.	List price:
QIAamp DNA Micro Kit (50) For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)		56304	$5457.0 MXN

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The QIAamp DNA Micro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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QIAamp DNA Micro procedure.|Laser microdissection PCR.|Efficient purification of DNA from small sample sizes.|

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.|DNA was purified from rodent kidney laser microdissection (LMD) samples using the QIAamp DNA Micro Kit or using a direct lysis PCR protocol, as indicated. GAPDH PCR was performed on 1 and 10 µl of each eluate. M: Marker; +: positive control; –: negative control.|DNA was purified from blood samples (1 –64 µl) using the QIAamp DNA Micro Kit following the blood protocol. DNA was eluted in 20 µl Buffer AE and 2 µl of each eluate was amplified by real-time PCR. Efficient amplification was shown in all cases.|

Performance

The resulting purified DNA is free of proteins, nucleases, and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure "Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure "Laser microdissection PCR"). DNA purified with the QIAamp DNA Micro Kit may also be used in short-tandem repeat (STR) genotyping, single-nucleotide pylomorphism (SNP) genotyping, or pharmacogenomic research.

Principle

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. DNA binds specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.

QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

Procedure

The QIAamp DNA Micro Kit simplifies isolation of DNA from small samples with a fast spin-column procedure (see flowchart "Procedure"). The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine, or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue, and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.

Applications

The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including:

Small volumes of blood
Blood cards
Urine
Small tissue samples, including laster microdissections

Feature	Specifications
Applications	Real-time PCR, STR analysis, LMD-PCR
Elution volume	20–100 µl
Format	Spin column
Main sample type	Whole blood
Processing	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA, mitochondrial DNA
Sample amount	1–100 µl
Technology	Silica technology
Time per run or per prep	30 minutes
Yield	<3 µg

Brochures & Guides

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	Analyzing Genetic Differences - (EN) Second edition — innovative tools	Show details
	Product Profile - QIAamp® genomic DNA kits	Show details

FAQs

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	How do I safely inactivate biohazardous flow-through material? FAQ ID -12	View
	Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation? FAQ ID -1188	View
	Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit? FAQ ID -1547	View
	I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro FAQ ID - 3560	View
	Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473	View
	What do you recommend for the cleanup of genomic DNA (gDNA)? FAQ ID -618	View
	What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728	View
	What is the composition of buffer AE? FAQ ID -730	View
	Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA? FAQ ID -754	View
	What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761	View
	Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908	View
	Do you have a protocol for the isolation of genomic DNA from sperm? FAQ ID -909	View
	Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells? FAQ ID -926	View
	How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305	View

Supplementary Protocols

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	Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp® DNA Micro Kit - (EN)	Show details

Kit Handbooks

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	QIAamp DNA Micro Handbook For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA	Show details

User-Developed Protocols

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	Purification of genomic DNA from bones using the QIAamp® DNA Micro Kit - (EN)	Show details
	Purification of genomic DNA from cultured cells using the QIAamp® DNA Micro Kit - (EN)	Show details

Safety Data Sheets

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	MSDS QIAamp DNA Micro Kit (50)
	CofA

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