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BCR-ABL1 Mbcr RGQ RT-PCR Kit

For the quantification of BCR-ABL1 Mbcr b3a2 and b2a2 fusion gene transcripts, results alignment on the international scale, MMR and MR4.5 reporting

 

  • MMR and MR4.5 reporting
  • Results aligned on the International Scale (IS)
  • With an IS-MMR calibrator in every run to prevent intra- and inter-lab variation
  • High reliability using EAC technology and single plasmid standards
  • Ready-to-use solutions including all reagents for RT and RQ-PCR validated on Rotor-Gene Q 5Plex HRM

 

Cat No./ID: 670913
BCR-ABL1 Mbcr RGQ RT-PCR Kit (24)
For 24 reactions: Reverse transcriptase, RT Mix, High Positive RNA Control, Low Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL1 Single Plasmid Standards, Taq DNA polymerase, BCR-ABL1 Mbcr and ABL1 qPCR Master mix
The BCR-ABL1 Mbcr RGQ RT-PCR Kit is intended for research use only and is not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance

To ensure the highest sensitivity, BCR-ABL1 Mbcr RGQ RT-PCR Kit has been optimized to detect very low levels of BCR-ABL1 p210 b2a2 or b3a2 transcripts. The limit of detection (LOD) is 3.13 CNMbcr (corresponding to a LOD of 0.0031% with ABL1CN = 100 000).

Principle

BCR-ABL1 Mbcr RGQ RT-PCR Kit is a ready-to-use kit for the detection of BCR-ABL1 p210 b2a2 or b3a2 transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL1 p210 b2a2 or b3a2 transcripts, relative to ABL1 control gene expression, in total RNA. The kit provides high levels of specificity, sensitivity, and reproducibility. Each kit provides 4 standard dilutions for ABL1, and 5 standard dilutions for BCR-ABL1 Mbcr. Use of a single plasmid for BCR-ABL1 and ABL1, enables reproducible quantification of transcripts and limits variability. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations. The kit also includes an IS-MMR calibrator allowing conversion of results to the International Scale for accurate measurement of molecular response, major molecular response (MMR) and the MR4.5, also called deep molecular response (DMR). The IS-MMR calibrator is included within every run to prevent intra- and inter-lab variation. High and low positive controls RNA are also included to monitor the reverse transcription and amplification steps of ABL1 and BCR-ABL1 Mbcr during transcript quantification.

Procedure

The BCR-ABL1 Mbcr RGQ RT-PCR Kit provides a comprehensive workflow with results in less than 7 hours from the starting material: RNA extraction from whole blood, reverse transcription of total RNA into cDNA, followed by the amplification and detection of cDNA by real-time PCR on Rotor-Gene Q 5plex HRM, with sensitive enzymes. Using BCR-ABL1 Mbcr RGQ RT-PCR Kit allows detection and quantification of BCR-ABL1 p210 b2a2 or b3a2 and ABL1 transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The BCR-ABL1 Mbcr RGQ RT-PCR Kit enables sensitive and reliable detection and quantification of BCR-ABL1 p210 b2a2 or b3a2 transcripts, conversion of results to the international scale, MMR and MR4.5 reporting, and is intended for research use only.

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