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virotype SBV RT-PCR Kit

For detection of RNA from Schmallenberg virus in samples from ruminants

  • Rapid and specific detection of RNA from Schmallenberg virus
  • Easy-to-use single reaction mix containing controls
  • RT-PCR run time of less than 2 hours
  • Can be performed in one cycler run with other virotype RT-PCR assays
Effective April 1, 2018, product available exclusively from INDICAL BIOSCIENCE (formerly QIAGEN Leipzig). Learn more.



The virotype SBV RT-PCR Kit allows the safe detection of RNA from Schmallenberg virus (SBV) in samples from ruminants. The kit contains all of the reagents for real-time RT-PCR in a single ready-to-use reaction mix. It can be used with serum, blood, semen, and meconium swab samples, individual or pooled blood, serum, tissue samples, and samples from cell-culture supernatants.
Cat No./ID: 281603
virotype SBV RT-PCR Kit (24)
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For 24 reactions: SBV Mix, Positive Control, Negative Control
Effective April 1, 2018, product available exclusively from INDICAL
Cat No./ID: 281605
virotype SBV RT-PCR Kit (96)
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For 96 reactions: SBV Mix, Positive Control, Negative Control
Effective April 1, 2018, product available exclusively from INDICAL
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Sensitive detection of SBV RNA.

Using the virotype SBV RT-PCR Kit, real-time RT-PCR was performed in triplicate on a titration series of in vitro RNA. The results demonstrate a high correlation (99%) between the number of copies and the amount of amplification product in the range from 108 to 100 copies/well, and positive detection of the virus down to 10 copies/well.

Performance
virotype SBV RT-PCR Kit detects RNA from Schmallenberg virus. A titration series was performed with ten-fold dilutions from 108 to 10 copies/well of SBV RNA. The results demonstrate a high correlation (99%) between the number of copies and the amount of amplification product in the range from 108 to 100 copies/well, and positive detection of the virus down to 10 copies/well (see the figure Sensitive detection of SBV RNA).
Principle

The Schmallenberg virus SBV is an enveloped, single-strand RNA virus that is mainly transmitted by midges. This kit is a real-time RT-PCR kit for the detection of RNA from SBV. RT-PCR is based on the amplification of specific regions of the pathogen genome. It is a sensitive and specific tool for pathogen detection.

Real-time RT-PCR involves the reverse transcription of viral RNA and subsequent amplification of the cDNA by PCR. The amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterward.

Procedure

 

The kit contains a ready-to-use mix with all of the necessary reagents for the detection of SBV RNA and the required positive and negative controls. With this kit, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination.

 

RNA is extracted from the sample material (serum, blood, tissue, semen, meconium swabs, or cell-culture supernatants) using a suitable sample preparation kit. The extracted RNA is used for the RT-PCR, which is run on a thermal cycler.

Applications

The virotype SBV RT-PCR Kit is for detection of RNA from Schmallenberg virus (SBV) in samples from ruminants.

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