RNeasy Micro Kit

For purification of up to 45 µg total RNA from cell and tissue samples
  • Fast procedure delivering high-quality total RNA in minutes
  • Ready-to-use RNA for high performance in any downstream application
  • Consistent RNA yields from very small amounts of starting material
  • No phenol/chloroform extraction
  • No CsCl gradients, no LiCl or ethanol precipitation

The RNeasy Micro Kit is designed for purification of up to 45 μg RNA from small cell and tissue samples. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Micro Kit can be fully automated on the QIAcube Connect. For larger samples, the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

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产品 货号 目录价:
RNeasy Micro Kit (50)
50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers
74004
询价

RNeasy Micro Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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产量稳定,适用于敏感应用。|从活检针中纯化获得高品质总RNA。|RNeasy Micro实验流程。|高效的柱上基因组DNA去除。|从单细胞中可靠纯化RNA。|
5位不同的用户使用RNeasy Micro Kit,从2 mg图示大鼠组织中分离总RNA,重复两次。在ABI Sequence Detection System上使用QuantiTect Probe RT-PCR Kit及c-jun特异性引物和探针进行定量real-time RT-PCR。|使用19.5口径的活检针从兔肝脏中进行细针抽吸活检,样本立即置于RNAlater TissueProtect Tube内稳定。使用RNeasy Micro Kit分离总RNA。采用Agilent 2100 Bioanalyzer分析显示了高质量的RNA。|RNeasy Micro Kit可用于从较小样本中分离总RNA。优化的实验方案适用于显微切割组织、少量标准组织、富含纤维的组织及细胞的RNA分离。还可提供RNA回收和浓缩实验方案。|使用RNeasy Micro Kit从图示量的HeLa细胞中分离总RNA。在ABI Sequence Detection System上使用QuantiTect Probe PCR Kit及c-jun特异性引物和探针进行定量real-time PCR。在逆转录后(+RT)以及未经逆转录步骤直接(-RT)扩增各样本,以评估柱上DNA酶切效率。"-RT"样本中无信号表示未检测到DNA。在"-RT"结果中,仅有50,000个细胞数据点可见。|使用RNeasy Micro Kit从图示量的HeLa细胞中分离总RNA。[A] 扩增曲线。[B] 交叉点(CP)值与细胞数的相关性。在LightCycler System上使用QuantiTect Probe RT-PCR Kit及β-actin特异性引物和探针进行real-time RT-PCR。|
性能
The RNeasy Micro Kit delivers highly reproducible yields of total RNA from small samples. Total RNA can reliably be purified from small numbers of cells, including a single cell, as well as from small amounts of standard tissues (see figures "Reliable RNA isolation from a single cell", "Highly reproducible yields for sensitive applications" and "High-quality total RNA from fine needle aspirates"). The RNeasy Micro Kit provides maximum sensitivity with precious samples in quantitative gene expression analyses, such as real-time RT-PCR, by efficient on-column digestion of genomic DNA (see figure "Efficient on-column removal of genomic DNA").
原理

The RNeasy Micro Kit is designed for isolation of total RNA from small samples such as microdissected tissues and fine needle aspirates, from milligram amounts of fibrous tissues including heart and muscle tissue, and from small numbers of cells down to single cells (e.g., FACS sorted cells). For microdissected FFPE tissues, we recommend the RNeasy FFPE Kit. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification and the unique design of RNeasy MinElute spin columns enables minimal elution volumes, as little as 10 µl, to concentrate the RNA. The small elution volume means that reaction volumes can be kept small in downstream applications, giving increased reaction efficiency. The high purity allows the complete sample to be used in downstream reactions, such as reverse transcription, without enzyme inhibition.

操作流程

The RNeasy Micro Kit is designed for isolation of total RNA from small samples. Samples (<5 mg tissue, or <5 x 105 cells) are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy MinElute spin column and RNA (up to 45 µg) binds to the silica membrane. RNase-free DNase, provided in the RNeasy Micro Kit, enables simple and efficient on-column digestion of genomic DNA for sensitive applications. Then DNase and any contaminants are efficiently washed away, and pure, concentrated RNA is eluted in 10–14 µl water.

应用

Highly reproducible isolation of RNA in a small elution volume (10 µl) using the RNeasy Micro Kit makes the highly pure RNA well-suited for sensitive quantitative gene expression analyses, including real-time RT-PCR starting with as little as one cell.

特点
参数
应用 PCR, qPCR, real-time RT-PCR, microarray
洗脱体积 10–14 µl
规格 Spin column
主要样本类型 Tissue, cells
处理 Manual
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 RNA
样本量 <5 mg
技术 Silica technology
每次运行或制备时间 30–40 minutes
产量 Varies

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试剂盒操作手册
1
For purification of total RNA from small samples, including
  • animal and human cells
  • animal and human tissues
  • fibrous tissues
  • microdissected cryosections
and for RNA cleanup and concentration
显示更多
参考文献
0
图片
Highly reproducible yields for sensitive applications.
产量稳定,适用于敏感应用。
5位不同的用户使用RNeasy Micro Kit,从2 mg图示大鼠组织中分离总RNA,重复两次。在ABI Sequence Detection System上使用QuantiTect Probe RT-PCR Kit及c-jun特异性引物和探针进行定量real-time RT-PCR。
High-quality total RNA from fine needle aspirates.
从活检针中纯化获得高品质总RNA。
使用19.5口径的活检针从兔肝脏中进行细针抽吸活检,样本立即置于RNAlater TissueProtect Tube内稳定。使用RNeasy Micro Kit分离总RNA。采用Agilent 2100 Bioanalyzer分析显示了高质量的RNA。
RNeasy Micro Procedure
RNeasy Micro实验流程。
RNeasy Micro Kit可用于从较小样本中分离总RNA。优化的实验方案适用于显微切割组织、少量标准组织、富含纤维的组织及细胞的RNA分离。还可提供RNA回收和浓缩实验方案。
Efficient on-column removal of genomic DNA.
高效的柱上基因组DNA去除。
使用RNeasy Micro Kit从图示量的HeLa细胞中分离总RNA。在ABI Sequence Detection System上使用QuantiTect Probe PCR Kit及c-jun特异性引物和探针进行定量real-time PCR。在逆转录后(+RT)以及未经逆转录步骤直接(-RT)扩增各样本,以评估柱上DNA酶切效率。"-RT"样本中无信号表示未检测到DNA。在"-RT"结果中,仅有50,000个细胞数据点可见。
Reliable RNA isolation from a single cell.
从单细胞中可靠纯化RNA。
使用RNeasy Micro Kit从图示量的HeLa细胞中分离总RNA。[A] 扩增曲线。[B] 交叉点(CP)值与细胞数的相关性。在LightCycler System上使用QuantiTect Probe RT-PCR Kit及β-actin特异性引物和探针进行real-time RT-PCR。