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QIAcube HT

Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
  • Reliable DNA, RNA and miRNA extraction from virtually any sample type
  • Mid- to high-throughput solution with dedicated purification kits saves costs and time
  • User-friendly software allows for easy data management and documentation
  • Innovative design features increase safety and minimize cross-contamination
  • Convenient, flexible and easy-to-use instrument with small footprint
 
QIAcube HT enables automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology. A variety of QIAcube HT Purification Kits lets users quickly and easily purify DNA, RNA and miRNA from almost any type of sample – including cells, tissues and stool samples, as well as from bacteria and viruses in animal samples. Automated protocols and dedicated accessories and consumables increase reliability and convenience and save valuable time. The system provides the same high-quality results as other trusted QIAGEN purification technologies.

Explore the virtual demo to learn more about QIAcube HT.
Cat No./ID: 9001793
QIAcube HT System
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack, installation and training, 1-year warranty on parts and labor
Cat No./ID: 9001899
QIAcube HT Priority Package
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack; includes Priority Package with installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Cat No./ID: 9001900
QIAcube HT Priority Package Plus
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack; includes Priority Package Plus with installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Cat No./ID: 9022649
Accessories Pack, QXT
For upgrading QIAxtractor instruments to process dedicated QIAcube HT kits: Transfer Carriage, Riser Block EMTR, and Channeling Adapter
The QIAcube HT is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
Analysis of genomic DNA purified from human blood.
Human blood from one donor was treated with sodium citrate, heparin, or EDTA. Genomic DNA was purified from a 50 or 200 µl aliquot of the treated blood manually with the QIAamp 96 DNA Blood Kit or on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit with the QIAamp 96 DNA QIAcube HT V1.QSP protocol. Purified DNA was eluted in 200 µl. PCR amplification of a 1.2 kb fragment from the single copy Hugl gene was carried out using QIAGEN HotStarTaq DNA Polymerase and 2.5 µl eluate in a total volume of 25 µl. A 1 µl aliquot of each PCR was analyzed on the QIAxcel Advanced with the QIAxcel DNA Screening Kit. M1: pUC18 HaeIII marker; M2: phiX174 HaeIII marker.
1
Automated purification of high-quality RNA from tissues.
RNA was isolated from 10 mg rat tissue (lung, kidney, heart, brain, spleen, and intestine) stabilized in RNAlater RNA Stabilization Reagent. Sample processing was automated on the QIAcube HT, BioRobot Universal System, or the QIAcube using QIAzol Lysis Reagent. A 1 µl aliquot of each purified RNA sample was analyzed on the QIAxcel Advanced using the QIAxcel RNA QC Kit v2.0 and the QX RNA Size Marker 200–6000 nt. The resulting high RNA integrity scores (RIS) indicate recovery of high-quality RNA.
2
Reliable automated purification.
Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.
3
Reliable purification from a range of sample types.
Various sample types were spiked with 20,000 IU of a typical DNA virus. Viral DNA was purified from 200 µl of each sample lysate using the QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol. Purified viral DNA was detected using an in-house PCR assay with 20 µl each eluate per reaction.
4
Efficient PCR inhibitor removal with all kits whether used manually or automated.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR was carried out on the Rotor-Gene Q using the QuantiTect Virus PCR Kit with 2 µl and 10 µl eluates respectively, from each sample in 20 µl reactions containing an internal control. CT values were compared to a standard containing no sample but only internal control (dark blue bar). All the three samples either extracted on different instruments or manually showed similar performance compared to the standard, regardless of the volume of eluate used, indicating no PCR inhibition.
5
Automated bacterial DNA purification from various animal stool samples gave comparable yields to the manual procedure.
Aliquots of 200 mg stool sample from different animals were processed on the QIAcube HT instrument (blue bars) using the QIAamp 96 PowerFecal QIAcube HT Kit, or manually using the QIAamp Fast DNA Stool Mini Kit (pink bars). Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either manually or on the QIAcube HT instrument showed similar performance for all the stool samples.
6
Co-isolation of viral RNA and bacterial DNA.
Consistent amounts of BVDV particles and Salmonella cells were simultaneously spiked into a variety of animal specimens. Samples were processed in parallel on the QIAcube HT with the cador Pathogen 96 QIAcube HT Kit or manually with the QIAamp cador Pathogen Mini Kit. Co-isolated viral RNA and bacterial DNA were identified using an in-house assay. Mean CT values are shown for [A] BVDV and [B] Salmonella. Small error bars (±1 SD of 3 extraction replicates) indicate high precision for both purification procedures.
7
Efficient and rapid automated DNA extraction.
The DNeasy mericon 96 QIAcube HT Kit has been adapted for rapid mid- to high-throughput purification of DNA from a variety of raw and processed food matrices on the QIAcube HT instrument. The modified cetyltrimethylammonium bromide (CTAB) extraction procedure allows DNA extraction from food samples in radically shorter time compared with conventional CTAB protocols.
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Highly repeatable viral RNA isolation.
Blood samples of various species were spiked with BVDV particles and were frozen at –20°C. Of each sample, 4 replicates were processed on QIAcube HT with the cador Pathogen 96 QIAcube HT Kit in 3 subsequent runs on different days. [A] Viral RNA was identified using an in-house assay, which included [B] an internal amplification control. Error bars represent ±1 SD of 3 replicates. The BVDV CT values for each set of quadruplicates showed a mean relative standard deviation of 0.67 ± 0.43%, and the inter-assay CV for each sample from 3 runs ranged from 0.14 to 0.89% (mean: 0.45%), indicating high repeatability of viral RNA purification on QIAcube HT.
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High performance in downstream assays.
Genomic DNA was purified from 200 µl human blood. Samples were processed with the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit and the QIAamp 96 DNA QIAcube HT V1.QSP protocol, or with the QIAcube using the QIAamp DNA Blood Mini Kit. Quantitative PCR of IL8 was carried out using the QuantiFast Probe Kit with 5 µL eluate in 25 µl. The CT values obtained show even amplification across the whole 96-well plate, indicating no well-to-well variation in DNA recovery with QIAcube HT. CT values were similar between the different purification platforms.
10
High sensitivity of purified viral nucleic acids in PCR and RT-PCR.
Nucleic acids were purified from serial dilutions of plasma samples spiked with a typical RNA or DNA virus. Samples were processed using QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol and were analyzed using in-house PCR and RT-PCR assays. The percentage of positive samples at low virus titers is shown. The resulting 95% probit values were 316.84 IU/ml for the RNA virus and 18.54 IU/ml for the DNA virus.
11
Linear recovery of total RNA from tissue lysates.
Lysates from rat lung tissue stabilized in RNAlater were serially diluted to produce samples containing equivalents of 2.5–25 mg tissue. Total RNA was purified from these samples using the QIAcube HT with the RNeasy QIAcube HT Kit and the RNeasy 96 QIAcube HT total RNA Tissue V1.QSP protocol. Purified RNA was eluted in 100 µl RNase-free water. Optical density measurements show a linear relationship between RNA recovery and amount of starting material.
12
Purification of DNA from complex food samples.
DNA was purified from orange juice, chocolate, and peanut butter using the QIAcube HT instrument and the DNeasy mericon 96 QIAcube HT Kit or manually using the DNeasy mericon Food Kit. Purified DNA was analyzed by qPCR of the trnL chloroplast tRNA gene using the QuantiFast SYBR® Green PCR Kit. The resulting CT values indicate similar performance from both purification methods. Diluting the input DNA 1:10 for qPCR analysis shows that all purified DNA was also free of PCR inhibitors.
13
Purification of genomic DNA from tissues.
Genomic DNA was purified from 5 mg rat lung, kidney, spleen, and liver tissue and 200 µl human blood. Samples were processed with the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit and the QIAamp 96 DNA QIAcube HT V1 QSP protocol or with the QIAcube using the QIAamp DNA Blood Mini Kit (blood) or the QIAamp DNA Mini Kit (tissues). Both platforms showed similar performance in [A] yield and [B] quality of purified DNA for each type of starting material.
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Reproducible 96-well RNA purification.
RNA was purified from 5 x 105 Jurkat cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit with the RNeasy 96 QIAcube HT total RNA cell with DNase V1.QSP protocol. Purified RNA was eluted in 110 µl RNase-free water. Quantitative RT-PCR of the human β-actin mRNA was carried out using the QuantiFast Probe RT-PCR Kit with 5 µl eluate in a total volume of 25 µl. The CT values obtained throughout one 96-well plate show even amplification across the whole plate, indicating no significant well-to-well variation in RNA recovery.
Performance
QIAcube HT provides fast and reliable automated high-throughput nucleic acid purification for use in a variety of sensitive downstream applications. Researchers can easily increase their sample purification throughput and expect the same high-quality results as from other trusted QIAGEN nucleic acid purification solutions.

DNA from blood, cells and tissues
Automated purification of total DNA from blood, cells and tissue samples is quick, easy and reliable with QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed (see figure Analysis of genomic DNA purified from human blood). QIAcube HT lets users increase sample purification throughput without having to compromise on quality or reliability, providing high yields of pure DNA that perform well in downstream analyses (see figures Purification of genomic DNA from tissues and High performance in downstream assays). 

DNA from stool samples
Automated purification of DNA from stool samples is quick, easy and reliable with QIAcube HT and the dedicated QIAamp 96 PowerFecal QIAcube HT Kit. Whether stool samples from 10 different animals were subjected to manual gDNA purification using the QIAamp Fast DNA Stool Mini Kit or automated using the dedicated instrument kit, the target could be successfully amplified in a real-time PCR assay, as indicated by comparable CT values (see figure Automated bacterial DNA purification from various animal stool samples gave comparable yields to the manual procedure).

Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples. The QIAamp 96 PowerFecal QIAcube HT Kit efficiently eliminates PCR inhibitors from samples, enabling reliable detection of DNA, similar to other QIAGEN stool DNA purification solutions whether used manually or automated (see figure Efficient PCR inhibitor removal with all kits whether used manually or automated).

Viral DNA and RNA from a variety of samples
The QIAamp 96 Virus QIAcube HT Kit enables automated purification of viral RNA and DNA from a broad range of sample types including fresh or frozen tissues, blood and other body fluids. The procedure yields high-quality viral nucleic acids that perform well in downstream PCR and RT-PCR analyses (see figure Reliable purification from a range of sample types). The procedure also provides high yields of pure RNA or DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figures Reliable automated purification and High sensitivity of viral nucleic acids in PCR and RT-PCR).

RNA from cells and tissues
RNA purification on QIAcube HT is simple and reliable with the RNeasy 96 QIAcube HT Kit and provides linear sample recovery with no significant well-to-well variation across a 96-well plate (see figures Linear recovery of total RNA from tissues and Reproducible 96-well RNA purification). This economical, easy-to-use system provides the same results as other trusted QIAGEN purification technologies (see figure Automated purification of high-quality RNA from tissues).  

Viral DNA and RNA and bacterial DNA from animal samples
QIAcube HT enables reliable purification of nucleic acids from undiluted animal whole blood with minimal risk of silica membrane clogging. Automated processing of samples in 96-well format is precise and repeatable between different purification runs performed on different days, providing not only convenience but also peace of mind (see figure Highly repeatable viral RNA isolation). Co-purification of viral RNA and bacterial DNA is easily automated with QIAcube HT and dedicated cador Pathogen QIAcube HT Kit, and provides the same high-quality nucleic acids as manual processing with the QIAamp cador Pathogen Mini Kit (see figure Co-isolation of viral RNA and bacterial DNA).  

DNA from complex food samples
The DNeasy mericon 96 QIAcube HT Kit has been specially adapted for rapid mid- to high-throughput purification of DNA from a variety of raw and processed food matrices on the QIAcube HT instrument. A modified cetyltrimethylammonium bromide (CTAB) extraction procedure allows DNA extraction from all food samples in radically shorter time compared with conventional CTAB protocols (see figure Efficient and rapid DNA extraction from complex food samples). Now users can prepare the same high-quality DNA as from the manual DNeasy mericon Food Kit with a quick and simple automated procedure (see figure Purification of DNA from complex food samples).

The DNeasy mericon Kit can only be used with the QIAcube HT Prep Manager Software when requesting a customized protocol. The protocol is not included in the standard configuration of the Prep Manager software. However, all DNeasy mericon Kit protocols are pre-installed and compatible with the QIAcube HT Operating Software 4.17.
Principle
Automated purification on the QIAcube HT is quick and cost-effective and delivers high-quality nucleic acids from 24–96 samples per run. Dedicated QIAcube HT Kits enable purification of nucleic acids from a diverse range of starting materials, including cells, tissues, bacteria, viruses, stool and food material. The QIAcube HT is extremely easy to use, and the intuitive software lets users start a run with just a few mouse clicks.

Dedicated QIAcube HT Kits for a wide range of starting materials
Specimen Input Kit Eluate Elution volume
Fresh, frozen or treated blood, cells and tissues Blood: up to 200 µl
Cells: up to 5 x 106 cells
Tissues: up to 20 mg
QIAamp 96 DNA QIAcube HT Kit (5) Total DNA 200 µl (default)
Fresh or frozen stool Up to 200 mg QIAamp 96 PowerFecal Kit (5) Host and bacterial DNA 100 µl (default)
Fresh or frozen tissues, swabs, blood and any other body fluids Body fluids: up to 200 µl
Tissues: up to 20 mg
QIAamp 96 Virus QIAcube HT Kit (5) Viral DNA and RNA 100 µl (default)
Animal or human cells and tissues Cells: up to 5 x 106 cells
Tissues: up to 40 mg
RNeasy 96 QIAcube HT Kit (5) Total RNA including miRNA 110 µl (default)
Animal samples including whole blood, serum, plasma, body fluids, swabs, washes, tissues, feces Body fluids: up to 200 µl
Tissues: up to 25 mg
cador Pathogen 96 QIAcube HT Kit (5) Viral RNA
Viral/bacterial DNA
150 µl (default)
Raw or processed food matrices Up to 300 mg food material DNeasy mericon 96 QIAcube HT Kit (5) DNA 200 µl (default)

QIAcube HT is a compact, benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. QIAcube HT comes with a laptop computer for added flexibility.

Advanced instrument safety features, including a translucent hood, help to protect precious samples from environmental contamination. The HEPA filter maintains positive clean air pressure on the worktable underneath the hood and protects precious samples from potential airborne contamination. The UV light provides efficient worktable decontamination and helps to prevent cross-contamination. QIAcube HT ejects used tips through the tip disposal chute into a disposable plastic box located at the side of the instrument. This external tip ejection prevents waste accumulation in the clean workspace. The tip disposal box holder keeps the box positioned correctly under the tip chute. The disposal box also comes with a secure-fitting lid to make waste disposal simple and safe.
Procedure
QIAcube HT provides automated, high-throughput nucleic acid purification for 24–96 samples per run, in increments of 8 samples. The instrument uses a vacuum-driven system to perform the simple and fast bind–wash–elute procedure during sample purification. The vacuum chamber is composed of two separate compartments. This configuration is one of many measures that minimizes sample cross-contamination during purification.

All materials needed for sample purification are placed onto the instrument worktable prior to the run. A 96-well sample block contains the starting sample material that will be lysed and transferred to the filter plate during the run. Easy-to-fill disposable troughs contain all necessary buffers and reagents for sample binding, washing, and elution. Pipet tips are positioned on the right side of the worktable.

Liquid-handling tasks are performed by the 8-channel pipetting head, which provides fast transfer of liquids during the run. The software tracks tip use and internally updates tip availability as the run proceeds. QIAcube HT optimizes the use of plasticware with a smart and economical tip reuse strategy. After washing, the pipetting head moves the 96-well filter plate to the elution compartment, where purified nucleic acids are eluted into the elution microtubes. The included 8-well strip caps securely close the elution microtubes for sample storage.
Software
QIAcube HT Software provides a user-friendly interface for operating the QIAcube HT instrument. The software wizard simulates the instrument worktable and makes it easy to set up and start a run with just a few mouse clicks. The software automatically calculates reagent volumes according to the number of samples, and fully documents every run. The software also allows you to import bar code databases, manage user access, and integrate Laboratory Information Management Systems (LIMS).
Features
Specifications
Accessories (supplied) Vacuum Control Station, Vacuum Chamber, Pinch Valve, Channeling Adapter (Channeling Block and Channeling Block Holder), Transfer Carriage, Riser Block EMTR, Adapter Reagent Plate SBS, Adapter Reagent Plate R1, Tip Disposal Box Holder, Tip Chute, Tip Chute Protective Cap, Waste Bottle with Lid Assembly
Communications RS232 and USB
Consumables (supplied) QIAcube HT Plasticware (5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad), 3x Tip Disposal Box with Lid, 2x Reagent Trough Lid 170 ml, 3x Reagent Trough with Lid 70 ml
Dimensions Instrument width: 540 mm (21 in.), height: 450 mm (18 in.), depth: 630 mm (25 in.); vacuum control station width: 495 mm (19 in.), height: 520 mm (20 in.), depth: 400 mm (16 in.)
Electrical 100–240 V AC, 250 VA, 50–60 Hz, overvoltage category II; mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages
HEPA filter Preinstalled dual-cartridge 3M Air-Mate HEPA filter system provides positive pressure clean air within the closed hood.
Humidity Operating humidity 40-70%
Notebook (supplied) QIAcube HT comes with a notebook computer for added flexibility.
Pipetting system 8 pipetting channels, pipetting volume (20-200 µl)
Place of operation For indoor use only
Pollution level 2
Precision 20-200 µl, less than 4% coefficient of variation
Software Supplied with unlimited user license with free upgrades by web download
Table capacity 4 x SBS positions (127.76 x 85.48 mm), compatible with 96-well plates or tip racks 1 custom adapter for a reagent trough plate compatible to accept reagent troughs vacuum chamber with chambers for waste and elution
Throughput 24-96 samples in increments of 8 samples
Tip disposal All used tips ejected externally; no waste accumulation on the worktable
UV lamp Preinstalled UV lamp for worktable decontamination
Warranty 1-3 years warranty, depending on service package
Weight QIAcube HT instrument: 41 kg (90 lb.); vacuum control station: 19 kg (42 lb.)

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Brochures & Guides (1)
For fast and reliable automated 96-well nucleic acid purification
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Instrument User Manuals (4)
Important note regarding new QIAcube HT declaration of conformity and instrument symbols
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Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Prep Manager Software.
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Protocol Files (2)
Procedure for installing Q Protocols for QIAcube HT Kits on the QIAcube HT and the QIAxtractor. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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These Q Protocols and installation files can only be used with QIAcube HT Operating software version 4.17 and are not compatible with the new QIAcube HT Prep Manager software.

QIAcube HT Prep Manager Software already includes all standard protocols. Additional protocols and kit configurations can be easily installed. Check for available updates under the "Operating Software" section of the "Product Resources" tab.
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Operating Software (3)
Files necessary for configuring the QIAcube HT Prep Manager Software to process QIAamp 96 PowerFecal QIAcube HT Kits. Not compatible with QIAcube HT Operating Software 4.17.

For kit configuration installation instructions, please refer to the QIAcube HT User Manual  chapter 4.4.5.
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QIAcube HT instrument operating software. We recommend installing the new improved QIAcube HT Prep Manager Software, which offers a state-of-the-art user-interface and a wizard-guided experimental setup for quick and easy purification experiments.

Important: It is not possible to install both QIAcube HT Operating Software 4.17 and QIAcube HT Prep Manager Software on the same computer. Please uninstall Prep Manager Software before installing QIAcube HT Operating Software 4.17. For more information, contact QIAGEN Technical Services.
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QIAcube HT instrument operating software. This software offers a state-of-the-art user interface and a wizard-guided experimental setup for quick and easy purification experiments.

For software installation instructions, please refer to the QIAcube HT Unpacking and Installation Instructions or to the QIAcube HT User Manual, chapter 4.4.1.

Important: It is not possible to install both QIAcube HT Prep Manager Software and QIAcube HT Operating Software 4.17 on the same computer. Please uninstall QIAcube HT Operating Software 4.17 before installing QIAcube HT Prep Manager Software. For more information, contact QIAGEN Technical Services.
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Safety Data Sheets (1)
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