Whole blood samples from various animals were spiked with RNA virus particles. The samples were processed in triplicate on 3 separate days. The data from the RT-PCR show high repeatability with error bars representing ±1 S.D.
Various animal samples were spiked with Gram-negative or Gram-positive bacteria. If necessary, the appropriate pretreatments were applied (B1 for bovine blood, B2 for swab medium, and an adapted version of B2 for bovine feces). The data from the PCR shows that the isolation of bacterial DNA was successful in all cases. The error bars represent ±1 S.D. from 3 QIAcube runs.
Bovine serum was spiked with RNA virus particles and the isolation protocol was run manually and on the QIAcube. The RT-PCR amplification data for the purification duplicates show high and identical isolation efficiency and linearity. Gray lines: manual isolation. Blue lines: automated isolation. Red lines: internal control.