Cat. No. / ID: 9002123
Cat. No. / ID: 9002874
Cat. No. / ID: 9002124
Cat. No. / ID: 9002125
Cat. No. / ID: 9002200
Cat. No. / ID: 9001941
Cat. No. / ID: 9021165
Cat. No. / ID: 9021163
The revolutionary QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA — streamlining workflows and reducing time to result. The QIAxcel Advanced system fully automates sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. DNA fragment analysis of 12 samples can be performed in as little as 3 minutes. Ready-to-run gel cartridges allow 96 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for tedious gel preparation. User-friendly QIAxcel ScreenGel software ensures convenient analysis and documentation of data. The QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement of the analyzed samples and allows easy interpretation of sample integrity.
The QIAxcel Advanced system not only speeds up DNA and RNA analysis by eliminating slab-gel analysis, but also contributes to streamlining the entire sample purification and analysis workflow. Used in combination with proven QIAGEN end-point PCR kits, the QIAxcel Advanced provides an all-in-one, pretested solution for reliable analysis of PCR fragments, ensuring reproducibility and significant time and cost savings.
One instrument, many applications
The QIAxcel Advanced system is a versatile solution for electrophoresis and offers a broad range of applications. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids – including single or multiple PCR fragments, DNA digested with restriction endonucleases, total RNA, and cRNA. Unlike other commercially available devices that provide processing of only up to 12 samples at time, the QIAxcel Advanced system allows processing of all 96 samples without manual intervention.
In genotyping applications, the QIAxcel Advanced system enables greater sizing accuracy and more sensitive detection than conventional agarose gel electrophoresis (see figure Accurate, high-throughput genotyping of bacteria). QIAxcel Advanced also provides a straightforward and effective means for checking the quality of genomic DNA (gDNA) samples prior to molecular screening and downstream assays (see figure Assessment of DNA degraded by sonication).
RNA analysis – objective quality values with the RNA integrity score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.
Such careful monitoring of the initial RNA sample, as well as products generated throughout the entire procedure, is crucial in microarray analysis, since RNA degradation strongly influences the predictive power obtained from the microarray data. The QIAxcel Advanced System is highly suitable for analyzing the integrity of total RNA and size distribution of fragmented or intact cRNA (see figure Streamlined RNA analysis using the QIAxcel system), and the throughput capacity makes it very convenient for laboratories employing 96-well RNA purification technologies that need a fast solution for quality control.
The QIAxcel Advanced instrument includes an array of light-emitting diodes and micro-optical collectors that latch to capillaries within QIAxcel gel cartridges. Fragments that are migrating through a gel matrix within the capillary pass excitation and detection spots and the signal is transmitted through a photomultiplier tube to the QIAxcel ScreenGel software for data interpretation (see figure Sample separation process).
The high detection sensitivity provided by the QIAxcel Advanced instrument enables robust results even with low concentrations of nucleic acid. With a resolution of 3–5 bp for fragments smaller than 0.5 kb, the QIAxcel Advanced system ensures greater accuracy than slab-gel methods, as well as greater confidence in data interpretation. Sample consumption is less than 0.1 μl per analysis, saving precious samples for further downstream analysis. Hands-free sample loading and self-contained components minimize exposure to hazardous chemicals, such as ethidium bromide (see figure Ready-to-run gel cartridges).
A range of QIAxcel Kits is available for a variety of applications, meeting different resolution and speed requirements (see table below).
|QIAxcel Kit||Analyte||Size range||100 bp – 500 bp*||500 bp – 1 kb*||1 kb – 5 kb*||5 kb – 10 kb*||Run time/
|QIAxcel DNA High Resolution Kit||DNA||15 bp–10 kb||3–5 bp||50 bp||200–500 bp||1–1.5 kb||7–20 min|
|QIAxcel DNA Screening Kit||DNA||15 bp–5 kb||20–50 bp||50–100 bp||500 bp||–||5 min|
|QIAxcel DNA Fast Analysis Kit||DNA||15 bp–3 kb||50–100 bp||100–250 bp||250 bp – 1 kb‡||–||3–5 min|
|QIAxcel RNA QC Kit v2.0||RNA||15 b–6 kb||–||–||–||–||10 min|
The QIAxcel Advanced System offers a broad range of optimized applications for DNA and RNA analysis and is highly suited for research in academic as well as pharmaceutical, biotechnology, and biomedical laboratories. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, synthesized oligonucleotides, total RNA, and cRNA.
The QIAxcel Advanced System has been used for applications such as:
QIAxcel ScreenGel software provides a new level of convenience
QIAxcel ScreenGel software, specifically developed for use with the QIAxcel Advanced system, is a powerful and user-friendly tool for data collection and analysis. Interactive tools simplify analysis, facilitate rapid data interpretation, and provide flexibility with data and results displayed in both electropherogram and gel image formats. Results can be viewed individually or displayed as overlay views for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation. A unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the size, height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet individual documentation needs. Complete process profiles for standardized sample processing — from running samples to data analysis, generating reports and exporting data — minimize the need for additional training for users (see figure Simplified and standardized data collection and analysis).
Easy setup and start
The Process Wizard simplifies experiment startup (see figure Easy-to-use software features). This convenient feature enables guided setup and allows run parameters to be defined and DNA size markers to be selected. Reagent lot number information can be included. Samples can be selected with a single click of the mouse and run checks can also be easily performed.
New RNA integrity score
The updated QIAxcel ScreenGel software now features the QIAGEN RNA Integrity Score (RIS), which enables objective quality measurement of analyzed samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.
Support of 21 CFR Part 11 compliance
QIAxcel ScreenGel software includes specific features that support the technical requirements of 21 CFR Part 11 regulations. Security features such as the following are available:
Different user profiles are available (Routine, Basic, Advanced, Admin) and are password protected (user login is required) for increased security. A simplified interface minimizes the need for user training and makes the software especially attractive for inexperienced users.
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Cat. No. / ID: 9242699
Cat. No. / ID: 9245252
Cat. No. / ID: 9241224A
For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software
Please follow the instructions described in the manual and run the DNA marker and the Calibration marker with the new method.
Make sure the Gel Cartridge has been calibrated with the computer currently connected. If not, please re-calibrate the Gel Cartridge.
Make sure the cartridge has equilibrated to room temperature (20°–25°C [68°– 77°F]) and stood upright in the cartridge stand for 20 min prior to use. Empty the buffer tray.
Wash the buffer tray in warm water and rinse thoroughly with deionized or reverse-osmosis water. Refill the buffer tray. Perform a control run with the alignment marker and bland samples (10 μl QX DNA Dilution Buffer). The marker bands should now appear within the chosen separation time.
Refer to Appendix D of the QIAxcel DNA Handbook for hot water soaking and gel droplet testing. The handbook can be accessed by going to the Resources tab in the link below:
The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.
If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.
The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.
The revolutionary QIAxcel Advanced System replaces traditional, labor-intensive gel analysis of DNA and RNA.
See: http://www.qiagen.com/Knowledge-and-Support/Videos-and-Virtual-Demos/Assay-Demos/QIAxcelAdvancedDemo/ for a virtual demonstration of the special software, features, and applications of the QIAxcel Advanced System.
When using this specific marker combination with the QIAxcel System or QIAxcel Advanced, i.e. for STR analysis, the 1.8 kb fragment lies outside of the range of detectable fragments and does not show up on the electropherogram and/or the gel view image.
N2 cylinders on the QIAxcel Advanced should be changed when one or both low pressure warnings are shown in the "Status Information" panel.
The left panel of the screen in the "Process" environment is called the "Status Information" panel.
"Pressure 1" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is sufficient for the current sample run; however, the N2 cylinder should be replaced once the run has finished.
"Pressure 2" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is insufficient for the current sample run and the analysis will not be performed. The N2 cylinder should be replaced.