FlexiGene DNA Kit

The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.

DNA purified using the FlexiGene system is sized up to 150 kb (see figure " High-quality genomic DNA"). It performs well in amplification and Southern blotting experiments, and is also suitable for archiving (see figure " Efficient downstream PCR analysis").

DNA is purified from 0.1–20 ml (blood), 0.1–2 ml (buffy coat), or 1–2 x 10⁶ cells (cultured cells) in a single tube, processed by centrifugation. Twelve samples can be processed in 1 hour. Depending on sample size, the rehydration volume is 0.2–1 ml.

DNA yields purified from different sample types using the FlexiGene procedure

* Whole blood with normal white blood cell counts (7x10⁶ cells/ml blood).

Sample	Volume	DNA yield (µg)
Blood*	0.3 ml	11–14
Blood*	2.0 ml	75–90
Blood*	10.0 ml	380–460
Buffy coat	0.5 ml	110–130
HeLa cells	106 cells	25–35

The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.

DNA purified using the FlexiGene DNA kit performs well downstream applications, including,

• PCR
• Southern blotting
• Archiving