QIAsymphony Virus/Bacteria Kits

For automated purification of viral nucleic acids or bacterial DNA from 1–96 samples

Features

  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents
  • Optimized protocols provide high nucleic acid yields
  • Novel, prefilled reagent cartridges for ease of use

Products

QIAsymphony Virus/Bacteria Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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QIAsymphony Virus/Bacteria Midi Kit (96)

Cat. No. / ID: 931055

For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories
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QIAsymphony Virus/Bacteria Mini Kit (192)

Cat. No. / ID: 931036

For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessories

Product Details

QIAsymphony Virus/Bacteria Kits provide automated purification of viral nucleic acids and bacterial DNA from a broad range of sample types on the QIAsymphony SP. For example, nucleic acids can be purified from plasma, serum, CSF, respiratory samples, urine, urogenital swabs, and transport media. Kits are available in mini and midi format for sample volumes of 200 μl and up to 1000 μl. Optimized protocols provide high yields of pure nucleic acids, and flexible elution volumes allow nucleic acid concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Performance

QIAsymphony Virus/Bacteria Kits enable robust and reproducible capture of nucleic acids. Purified viral and bacterial nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications. Efficient yields and flexible elution volumes (60 μl, 85 μl, or 110 μl) to suit the requirements of downstream applications ensure sensitive analytical detection (see figure " Sensitive detection of Chlamydia trachomatis in urine").
See figures

Principle

QIAsymphony Virus/Bacteria Kits combine the speed and efficiency of silica-based purification of viral and bacterial nucleic acids with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
See figures

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.

QIAsymphony Virus/Bacteria protocols
Protocol Kit  Sample material Processed volume
Cellfree 200 QIAsymphony Virus/Bacteria Mini Kit (192) Plasma, serum, and CSF 200 μl
Cellfree 500   QIAsymphony Virus/Bacteria Midi Kit (96)  Plasma, serum, and CSF 500 μl
Cellfree 1000   QIAsymphony Virus/Bacteria Midi Kit (96)  Plasma, serum, and CSF 1000 μl
Complex 200 QIAsymphony Virus/Bacteria Mini Kit (192) Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)  200 μl
Complex 400 QIAsymphony Virus/Bacteria Midi Kit (96) Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) 400 μl 
Complex 800 QIAsymphony Virus/Bacteria Midi Kit (96) Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) 800 μl 

Applications

QIAsymphony Virus/Bacteria Kits enable highly sensitive detection of a broad range of DNA and RNA viruses, as well as bacterial pathogens. Yields are linear, allowing accurate quantitative analysis for both low and high viral or bacterial titers. The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time PCR
Elution volume60-110 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinPurification of viral RNA and DNA and bacterial DNA
Input volume800 µl (Pathogen Complex Protocol); 1000 µl (Virus Cellfree Protocol)
TechnologyMagnetic particles
Sample typesSerum, plasma, CSF, cell-free body fluids, fresh or frozen material (body fluids, swabs, secretions, tissues, stool specimens)

Resources

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Labware Files and Documents (1)
For use with software version 5.0 or higher 
Application/Protocol Documents (8)
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
Kit Handbooks (1)

FAQ

Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

No. The use of internal controls with the QIAsymphony Virus/Bacteria Kits is not mandatory. When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 µl).

 

FAQ ID -2040
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?

The QIAsymphony Virus/Bacteria Mini Kit is used with the 'Cellfree_200' and 'Complex_200' protocols.

The QIAsymphony Virus/Bacteria Midi Kit is used with the 'Cellfree_500’, 'Cellfree_1000', ‘Complex_400’ and 'Complex_800' protocols.

FAQ ID -2038
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?

Plasma, serum, and CSF samples can be processed using the Virus Cellfree protocols of the QIAsymphony Virus/Bacteria Kits. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the Pathogen Complex protocols.

 

FAQ ID -2036
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
Plasma, serum, and CSF samples can be processed using the QIAsymphony DSP Virus/Pathogen cellfree protocols. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the pathogen complex protocols.
FAQ ID -2928
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?

Sample volumes that can be processed with the QIAsymphony Virus/Bacteria Kits depend on the protocol:

  • 'Cellfree_200' and 'Complex_200':  200 µl
  • 'Complex_800':                            800 µl
  • 'Cellfree_1000':                           1000 µl

 

FAQ ID -2037
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?

Pre-treatment of samples used with the QIAsymphony Virus/Bacteria Kits can improve nucleic acid extraction for some sample types. Viscous samples (e.g., sputum, respiratory aspirates) might require liquefaction to enable pipetting. Pretreatment protocols are provided in the QIAsymphony Virus/Bacteria Handbook.

 

FAQ ID -2039