Find out which OEM master mix fits your assay
Get the quantitative/ real-time (qRT/RT) PCR master mixes from OEM by QIAGEN and try out our four proven products. Accelerate your innovation by saving time on trial and error in the development of your PCR assay. Avoid having to start from scratch; simplify your R&D process by testing our pre-formulated products. We have selected one and two -step RT-PCR mixes with R&D simplification in mind. This way, you can quickly test the various options and ascertain which of our RT-PCR kits works best for you.Four RT-PCR kits - ready for you
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QuantiNova Pathogen + ICFast; simultaneous detection of viral RNA/DNA and bacterial DNA; works with degenerated primer/probe sets
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QuantiNova Multiplex RT-PCR KitHighly sensitive, quantitative RT-qPCR for use with probes
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QuantiTect Multiplex RT-PCR NR KitHighly sensitive, quantitative RT-qPCR for use with probes; contains dUTP
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ZipScript™ One-Step RT-qPCR KitEfficient RNA dependent qPCR for a broad range of sample detection
Interested in OEM master mixes?
Allow the OEM by QIAGEN team to help you accelerate your innovation.
Pre-optimized kits – a quick solution to overcome your assay development challenges
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Reliable, robust performance- Proven RT-PCR mixes
- Successful in the market
- Familiar QIAGEN chemistries
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Established protocols- Formulations that are used by countless researchers and diagnostic labs
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Available as OEM- Bulk production
- Customized volume and packaging
- Reagents easily combined into your assays
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For overcoming R&D challenges- Sensitivity, specificity
- Reproducibility, scalability
- Ease of use
- Time to results
Successful OEM PCR mixes with proven benefits:
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QuantiNova Pathogen + IC |
QuantiNova Multiplex RT-PCR Kit |
QuantiTect Multiplex RT-PCR NR Kit |
ZipScript™ One-Step RT-qPCR Kit |
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|---|---|---|---|---|
| Short description |
Fast; simultaneous detection of viral RNA/DNA and bacterial DNA; works with degenerated primer/probe sets |
Highly sensitive, quantitative RT-qPCR for use with probes |
Highly sensitive, quantitative RT-qPCR for use with probes; contains dUTP |
Efficient RNA dependent qPCR for a broad range of sample detection |
| Application | Virus, bacteria, fungi detection |
Gene expression / quantification |
Gene expression / quantification |
General use RT-qPCR |
| Configuration | RT-PCR Master Mix, 4x polymerase and RT included in the same master mix |
PCR Master Mix, 4x w/ separate RT Mix, RT in a separate tube |
PCR Master Mix, 2x incl. dUTP w/ separate RT Mix, RT in a separate tube |
25X Enzyme Mix and 2X Reaction buffer, Polymerase and RT in one tube but buffers in a separate tube |
| Inhibitor Resistance |
Robust | Low | Low | Medium |
| Benchtop S/U | 2-Phase HotStart allows for room temp. reaction set up on bench for at least 1 hr |
2-Phase HotStart allows for room temp. reaction set up on bench for at least 1 hr |
NA | NA |
| Room temperature setup |
yes | NA | NA | Automation friendly |
| Visual pipetting control using Blue MasterMix and Yellow Template Dilution buffer (optional) |
yes | yes | NA | NA |
| Optimized for degenerated primers & probes |
yes | NA | NA | NA |
| PCR HotStart procedure |
Guard Mediated antibody | Guard Mediated antibody | Chemically inactivated | Antibody-mediated |
| Multiplexing (depends on assay design), higher multiplexing is possible |
4-plex | 5-plex | 3-plex | 5-plex |
| Temperature for cDNA Synthesis |
Warm Start RT @50C | Warm Start RT @50C | 42C to 50C | |
| Sensitivity | High | High - single cell | High - single cell | High - less than 1pg total human RNA |
| Shelf Life | 2-8C 6m 18m -20C |
2-8C 6m 18m -20C |
2-8C 6m 18m -20C |
24 months @-20C |
| dUTP included | no | no | yes | no |
| Internal Control provided (choice of HEX or CY5 Assay for detection) |
RNA and DNA IC | RNA IC | NA | NA |
Interested in OEM?
If you still require some tweaking after testing our master mixes, no problem!
A dedicated OEM project manager will be happy to discuss the required adjustments.
A dedicated OEM project manager will be happy to discuss the required adjustments.
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QuantiNova® Pathogen + IC
For ultrafast, simultaneous detection of viral RNA/DNA and bacterial DNA, including internal control
- Preoptimized internal controls
- 4-plex RT-PCR for detection of both pathogen targets and internal control
- Unique two-phase hot-start procedure for convenient room-temperature setup
- Stable RT included in the master mix
- One easy-to-use protocol
- Visual pipetting control
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QuantiNova Multiplex RT-PCR Kit
For highly sensitive and specific one-step qRT-PCR and multiplex qRT-PCR
- Unique two-phase hot-start procedure for room-temperature setup
- Visual pipetting control
- Internal control for positive in-process verification of successful RT-PCR
- Accurate quantification over several logs of template
- Sensitive detection of up to five targets in one tube
- qRT-PCR using sequence-specific probes or SYBR® Green
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QuantiTect Multiplex RT-PCR NR Kit
For one-step qRT-PCR and multiplex qRT-PCR
- Highly sensitive detection of low-copy targets
- qRT-PCR using sequence-specific probes or SYBR® Green
- Accurate quantification over several logs of template
- No need to optimize reaction and cycling conditions
- Detection of reference gene and up to three targets in the same tube
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ZipScript One-Step RT-qPCR Kit
For highly sensitive and specific one-step RT-PCR
- Fast and easy one-tube setup
- Efficient one-step RT-PCR of any RNA template without optimization
- Unique enzyme mix for high specificity and sensitivity
- Balanced mixture of enzymes with optimized reverse-transcription buffer
Learn about diverse OEM solutions
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Custom oligosHigh quality oligonucleotides, primer and probes manufactured to your specifications. -
Digital PCR systemsNucleic acid quantification technologies for manufacturers, QIAcuity is available as OEM. -
Nucleic acid purificationIf you have a sample preparation need, OEM by QIAGEN has a bulk solution for you.
