Find out which OEM master mix fits your assay
Get the quantitative/ real-time (qRT/RT) PCR master mixes from OEM by QIAGEN and try out our four proven products. Accelerate your innovation by saving time on trial and error in the development of your PCR assay. Avoid having to start from scratch; simplify your R&D process by testing our pre-formulated products. We have selected one and two -step RT-PCR mixes with R&D simplification in mind. This way, you can quickly test the various options and ascertain which of our RT-PCR kits works best for you.Four RT-PCR kits - ready for you
Interested in OEM master mixes?
Allow the OEM by QIAGEN team to help you accelerate your innovation.
Pre-optimized kits – a quick solution to overcome your assay development challenges
Successful OEM PCR mixes with proven benefits:
|
QuantiNova Pathogen + IC |
QuantiNova Multiplex RT-PCR Kit |
QuantiTect Multiplex RT-PCR NR Kit |
ZipScript™ One-Step RT-qPCR Kit |
|
|---|---|---|---|---|
| Short description |
Fast; simultaneous detection of viral RNA/DNA and bacterial DNA; works with degenerated primer/probe sets |
Highly sensitive, quantitative RT-qPCR for use with probes |
Highly sensitive, quantitative RT-qPCR for use with probes; contains dUTP |
Efficient RNA dependent qPCR for a broad range of sample detection |
| Application | Virus, bacteria, fungi detection |
Gene expression / quantification |
Gene expression / quantification |
General use RT-qPCR |
| Configuration | RT-PCR Master Mix, 4x polymerase and RT included in the same master mix |
PCR Master Mix, 4x w/ separate RT Mix, RT in a separate tube |
PCR Master Mix, 2x incl. dUTP w/ separate RT Mix, RT in a separate tube |
25X Enzyme Mix and 2X Reaction buffer, Polymerase and RT in one tube but buffers in a separate tube |
| Inhibitor Resistance |
Robust | Low | Low | Medium |
| Benchtop S/U | 2-Phase HotStart allows for room temp. reaction set up on bench for at least 1 hr |
2-Phase HotStart allows for room temp. reaction set up on bench for at least 1 hr |
NA | NA |
| Room temperature setup |
yes | NA | NA | Automation friendly |
| Visual pipetting control using Blue MasterMix and Yellow Template Dilution buffer (optional) |
yes | yes | NA | NA |
| Optimized for degenerated primers & probes |
yes | NA | NA | NA |
| PCR HotStart procedure |
Guard Mediated antibody | Guard Mediated antibody | Chemically inactivated | Antibody-mediated |
| Multiplexing (depends on assay design), higher multiplexing is possible |
4-plex | 5-plex | 3-plex | 5-plex |
| Temperature for cDNA Synthesis |
Warm Start RT @50C | Warm Start RT @50C | 42C to 50C | |
| Sensitivity | High | High - single cell | High - single cell | High - less than 1pg total human RNA |
| Shelf Life | 2-8C 6m 18m -20C |
2-8C 6m 18m -20C |
2-8C 6m 18m -20C |
24 months @-20C |
| dUTP included | no | no | yes | no |
| Internal Control provided (choice of HEX or CY5 Assay for detection) |
RNA and DNA IC | RNA IC | NA | NA |
Interested in OEM?
If you still require some tweaking after testing our master mixes, no problem!
A dedicated OEM project manager will be happy to discuss the required adjustments.
A dedicated OEM project manager will be happy to discuss the required adjustments.