Forensic laboratories face a critical decision when selecting between capillary electrophoresis (CE) and next-generation sequencing (NGS) chemistries for DNA analysis. CE is known for rapid, cost-effective STR genotyping, while NGS provides more comprehensive sample insight by detecting sequence variants. While NGS requires additional instrumentation and expertise, it is essential for challenging cases like unidentified human remains and can be comparable in cost.

This webinar will focus on assessing the effectiveness of CE and NGS in analyzing degraded and burned skeletal remains. The ForenSeq MainstAY Kit, ForenSeq mtDNA Whole Genome Kit, ForenSeq Kintelligence Kit and Investigator 24plex QS Kit will be evaluated. Degraded samples were created by thermally degrading femurs and extracting DNA using the EZ2 Connect Fx Extra-Large Volume Bone Protocol. Extracts were then screened via traditional qPCR and gel-based analysis on the QIAxcel Connect to assess DNA quality and quantity.

Dr. Houston will discuss the comparison of these methods and their implications for forensic DNA analysis, particularly in unidentified human remains cases. The webinar will also explore how these screening techniques can enhance forensic DNA analysis decision-making. Learn how these advancements can enhance your laboratory's forensic DNA analysis capabilities.