TissueLyser Adapter Sets

For efficient disruption of samples in 1.2 mL Collection Microtubes (racked), 2 mL tubes, 5 mL tubes, 50 mL tubes or 96-well plates on the TissueLyser III & II

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TissueLyser Adapter Set 2 x 24

Cat. No. / ID: 69982

Two sets of adapter plates and two racks for use with 2 mL microcentrifuge tubes on the TissueLyser III & II

Accessories

Tube Adapter Sets

Plate Adapter Sets

Holder Set

For

2 mL tubes

5 mL tubes

50 mL tubes

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TissueLyser Adapter Sets are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

TissueLyser Adapter sets are designed for efficient and user-friendly disruption of samples in 1.2 mL Collection Microtubes (racked), 2 mL tubes, 5 mL tubes, 50 mL tubes or 96-well plates on the TissueLyser III & II.

The TissueLyser Adapter Set 2 x 24 contains a pair of TissueLyser Reaction-Tube Holders, 24-Tube. Each holder holds up to 24 microcentrifuge tubes (2 mL), totaling up to 48 samples per run. The use of Safe-Lock tubes is strongly recommended (e.g., Eppendorf 2 mL Safe-Lock micro test tubes).

The TissueLyser Adapter Set 2 x 96 is for use with QIAGEN Collection Microtubes (racked) and Collection Microtube Caps, totaling up to 192 samples per run. Collection Microtubes are 1.2 mL in volume.

The 5 mL Tube Adapter Set is for use with 5 mL bead tubes (e.g., PowerWater DNA Bead Tubes). Each set holds up to 16 tubes.

The 50 mL Tube Adapter Set is for use with 50 mL tubes (e.g., 50 mL Falcon tubes). Each adapter can hold up to 4 tubes.

The Plate Adapter Set is for use with two 96-well plates; it is compatible with the PowerBead Pro Plates.

The 2 mL Tube Holder Set is for use with 2 mL tubes. It can only be used with the Plate Adapter Set. Each holder can fit up to 48 tubes.

Resources

Up to 1 x 10⁹ bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.

For high-throughput disruption of biological samples

Accessories for the simultaneous disruption of samples in tubes and 96-well plates on the TissueLyser III & II

FAQ

We do not recommend using the adapters of the TissueLyser II with liquid nitrogen. Instead, the adapters can be precooled at –80°C, either in the freezer or on dry ice.

FAQ ID -9173

TissueLyser Adapter Set 2 x 96 should be used with Collection Microtubes (racked) and Collection Microtube Caps (cat. nos. 19560 and 19566 respectively).

FAQ ID -3086

Yes, we have the following protocols:

Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).

FAQ ID -1550

100 mg tissue per sample tube is the maximum amount we recommend when using the 2 x 24 Adapter Set. Larger amounts of tissue would require considered optimization, depending on the tissue type and condition.

When using 100 mg tissue, for best homogenization results, we recommend cutting the sample into 2 pieces of approximately 50 mg each prior to disruption and performing disruption twice, each time at 25 Hz for 5 min.

FAQ ID -9178