Effectie tissue disruption.
Effective tissue disruption.
Intact protein suitable for all types of analysis.
Intact protein suitable for all types of analysis.
High-quality DNA from animal tissues.
High-quality DNA from animal tissues.
High-quality DNA from plant tissues.
High-quality DNA from plant tissues.
Intact RNA from plant tissues
Intact RNA from plant tissues.
ID_0191_LT
Pure RNA with high RIN values.
S_1565_GEF_TLLT_50
TissueLyser LT.
Various rat tissues were disrupted using the TissueLyser LT or TissueLyser II. [A] DNA was purified from 25 mg samples on the QIAcube using the DNeasy Blood & Tissue Kit. DNA yields were determined using a spectrophotometer. [B] RNA was purified from 20 mg samples on the QIAcube using the RNeasy Fibrous Tissue Mini Kit (skin, heart, and lung) or RNeasy Lipid Tissue Mini Kit (brain). RNA yields were determined using a spectrophotometer.
Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. Total protein was purified using the Qproteome Mammalian Protein Prep Kit and then analyzed by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. LT: TissueLyser LT; II: TissueLyser II; M: markers.
Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. DNA was purified on the QIAcube using the DNeasy Blood & Tissue Kit, and then used in PCR with the HotStarTaq Plus Master Mix Kit and a PGK1 primer system. The 120 bp PCR product was analyzed on the QIAxcel using the QIAxcel DNA High Resolution Kit. LT: TissueLyser LT; II: TissueLyser II; M: markers.
Various plant tissues (100 mg each) were disrupted in precooled adapters using the TissueLyser LT or TissueLyser II. DNA was purified on on the QIAcube using the DNeasy Plant Mini Kit and then analyzed on an agarose gel. LT: TissueLyser LT; II: TissueLyser II; M: markers.
Various plant tissues (100 mg each) were disrupted in duplicate in precooled adapters using the TissueLyser LT. RNA was purified using the RNeasy Plant Mini Kit and then analyzed on an agarose gel. The sharp ribosomal RNA bands indicate purification of intact RNA.
Rat heart and lung (20 mg each) were disrupted using the TissueLyser LT. RNA was purified on the QIAcube using the RNeasy Fibrous Tissue Mini Kit and then analyzed on the Agilent 2100 Bioanalyzer. The RIN values were 8.3 (heart) and 9 (lung).