Analysis of antibacterial resistance in Helicobacter pylori.
Pyrosequencing-based bacterial identification.
Time savings afforded by Pyrosequencing.
Analysis of mutations in the 23S genes that confer antibacterial resistance in
Helicobacter pylori
.
PCR primers were designed for conserved regions of 16S rRNA and the sequencing primer was placed immediately upstream of the hypervariable region V1 (blue). One set of PCR and sequencing primers is sufficient to discriminate the listed species (as well as others not included in the alignment).
From RNA isolation to data analysis, with Pyrosequencing, detection of resistance-conferring mutations in a RNA virus can easily be completed within a single workday. Times given reflect sequencing of a 44-base region in 96 viral samples.