More than quantification!
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Overview
- Interactive Workflow
Experience for yourself how easy it is to use QIAxpert
- The System
View the innovative technology and advanced bioinformatics
- Data
Get true insight into your sample
Touchscreen and Software
Touchscreen
QIAxpert features a full-color integrated touchscreen interface, so no extra computer is required. The intuitive and easy-to-use QIAxpert Software was designed to fit with a busy lab schedule.
Touchscreen and Software
Analysis protocols
QIAxpert performs classic nucleic acid and protein quantification based on absorbance measurements. In addition, smart analysis protocols called “apps” come pre-installed to quantify specific analytes of interest. Based on top bioinformatics expertise, these spectral profiling protocols let users focus on the molecule of interest, subtracting other absorbing impurities and background turbidity.
Touchscreen and Software
Result display
The measurement result screen shows detailed results for individual samples and allows for quick review of sample quality and quantity.
Touchscreen and Software
Report export
A comprehensive report along with other data files can be easily exported via a USB stick or network drive.
Touchscreen and Software
View previous runs
After sample measurement, the data are stored on the instrument and can be accessed at any time from the experiment list.
Touchscreen and Software
User manager
Multiple users can be set up and a user selected prior to starting a measurement to ensure accurate data recording.
Sample carrier
Watch the video to learn about the benefits provided by the microfluidic QIAxpert slides. Along with minimal sample volume requirements, no cleaning is needed between runs, and there is no risk of cross-contamination.
Operation
Watch how simple it is to operate the QIAxpert. Samples are easily loaded, and results are displayed in less than 2 minutes.
Performance data
Reproducibility
The QIAxpert provides highly reproducible results over a wide dynamic range. In this experiment, 5 replicates of each sample from a dilution series of salmon sperm DNA were measured on the QIAxpert. Reproducibility was assessed by calculating the coefficient of variation.
Performance data
Linearity
To assess the linearity of the system, pure gDNA was serially diluted to generate samples from 2000 to 2 ng. The resulting dilutions were then measured on the QIAxpert using 2 µl for each of 5 replicates. The quantifications from the QIAxpert were plotted against the known concentration values. The resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAxpert up to 2000 ng/ml or 40 OD (A260, 10 mm).
DNA
Spectral content profiling
Quantification of dsDNA purified with QIAamp silica membrane technology is shown. The concentration was determined based on the absorbance at 260 nm and using a factor of 50.
The blue curve shows the DNA portion measured. Other absorbing impurities and nonDNA nucleic acids are subtracted from the result (orange curve) and the background is checked for turbidities (none in this example), which would be represented as a gray curve. The yellow line is the residual spectrum, which is the part of the measured spectrum that cannot be attributed to the reference profiles used in the algorithm.
The blue curve shows the DNA portion measured. Other absorbing impurities and nonDNA nucleic acids are subtracted from the result (orange curve) and the background is checked for turbidities (none in this example), which would be represented as a gray curve. The yellow line is the residual spectrum, which is the part of the measured spectrum that cannot be attributed to the reference profiles used in the algorithm.
DNA
UV/VIS scan
Results for dsDNA measured with the A260 dsDNA app are shown. This app quantifies dsDNA based on the total absorbance at 260 nm using the extinction coefficient for dsDNA of 50 µl/ng*cm.
RNA
UV/VIS scan
Results for total RNA measured with the A260 RNA app are shown. This app quantifies total RNA based on the total absorbance at 260 nm using the molar extinction coefficient for RNA of 40 µl/ng*cm.
RNA
Spectral content profiling
Results of quantification of total RNA purified with RNeasy technology are shown. The concentration was determined based on the absorbance at 260 nm using the molar extinction coefficient for RNA of 40 µl/ng*cm.
The blue curve shows the RNA portion measured. Other absorbing impurities and nonRNA nucleic acids are subtracted from the result (orange curve) and the background is checked for turbidities (gray curve). The yellow line is the residual spectrum, which is the part of the measured spectrum that cannot be attributed to the reference profiles used in the algorithm.
The blue curve shows the RNA portion measured. Other absorbing impurities and nonRNA nucleic acids are subtracted from the result (orange curve) and the background is checked for turbidities (gray curve). The yellow line is the residual spectrum, which is the part of the measured spectrum that cannot be attributed to the reference profiles used in the algorithm.
Protein
Results for purified protein measured with the A280 Protein app are shown. This app quantifies purified protein based on the total absorbance at 280 nm and using extinction coefficients entered prior to result analysis.
Get in touch!
If you still have any questions, contact us:www.qiagen.com/about-us/contact/
The QIAxpert is already attracting fans!
"We've been working with the QIAxpert spectrophotometer for half a year and are extremely satisfied with the easy-to-use system."
Dr. Sebastian Bartels, University Hospital Freiburg, Germany
"It has automatic and very comprehensive data export, and the possibility to measure 16 samples in parallel saves us a lot of time and frustration."
Dr. Sebastian Bartels, University Hospital Freiburg, Germany
"We highly recommend the QIAxpert."
Dr. Sebastian Bartels, University Hospital Freiburg, Germany
