Empowering Single-Cell Research

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Concept

The QIAscout device together with its array is an affordable and accessible instrument enabling you to isolate and recover single cells.

  • Ideal for a variety of eukaryotic cells
  • Works in conjunction with inverted microscopes
  • Cells remain viable after separation
  • Isolated cells can be used for direct analysis or clonal expansion

Setup and Use

Setting up the QIAscout is easy and just takes minutes! Follow the guide in the video to see how easy it is to get your cells of interest.

Choose Objective

  • If your objective is not listed above, check if your objective diameter (ØD) is between 18–30 mm to be compatible with the QIAscout release device. Refer to the table below the image to select the right release device insert based on your objective dimensions.

    *All pictures shown here are for illustration purpose only.

  • The following table shows which insert to use based on the diameter of your microscope objective.

    Parameters Dimensions Insert required
    W.D. (mm)
    Working Distance
    10 - 19
    ØD (mm)
    Objective Diameter
    18 - 30 18 - 20 = #1
    21 - 23 = #2
    23 - 25 = #3
    25 - 27 = #4
    L (mm) Min. 28
    Objective
    Magnification
    4x, 5x or 10x

Choose Objective

Result

Mechanical Stage 130x85 R/L with short coaxial drive - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

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*All pictures shown here are for illustration purpose only.

Result

Scanning Stage 130x100 STEP (D) - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Mechanical Stage 130x85 R/L with short coaxial drive - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Mechanical Stage 130x85 R/L with short coaxial drive - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Mechanical Stage 130x85 R/L with short coaxial drive - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Mechanical Stage 130x85 R/L with short coaxial drive - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Mechanical Stage 130x85 R/L with short coaxial drive - fit

Tips: Required spacer rings are available for the stage
Stage insert, metal D=115 mm fits, glass D=115 mm doesn't fit

Universal Mounting Frame K

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Choose Objective

  • If your objective is not listed above, check if your objective diameter (ØD) is between 18–30 mm to be compatible with the QIAscout release device. Refer to the table below the image to select the right release device insert based on your objective dimensions.

    *All pictures shown here are for illustration purpose only.

  • The following table shows which insert to use based on the diameter of your microscope objective.

    Parameters Dimensions Insert required
    W.D. (mm)
    Working Distance
    10 - 19
    ØD (mm)
    Objective Diameter
    18 - 30 18 - 20 = #1
    21 - 23 = #2
    23 - 25 = #3
    25 - 27 = #4
    L (mm) Min. 28
    Objective
    Magnification
    4x, 5x or 10x

Result

Fits

Tips: Mechanical stage, stroke: 126 (X) x 78 (Y) mm

Terasaki Holder (for 65 mm dish)
C-S-HT Terasaki Holder for Terasaki holder and φ65 dish
C-S-HU Universal Holder for Terasaki plate holder, glass slide, φ35-65 dish and hemocytometer

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: Mechanical stage, stroke: 126 (X) x 78 (Y) mm

Terasaki Holder (for 65 mm dish)
C-S-HT Terasaki Holder for Terasaki holder and φ65 dish
C-S-HU Universal Holder for Terasaki plate holder, glass slide, φ35-65 dish and hemocytometer

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Choose Objective

Fits, if r>4.2 cm

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Fits, if r>4.2 cm

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Fits, if r>4.2 cm

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Fits, if r>4.2 cm

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Result

Fits

Tips: DMi1 and DM IL LED: Microscope-specific stage works; install object guide for fixed stages
DMi8: Change of stage would be necessary (e.g., LMT200 high precision stage)

Universal Holding Frame M

Is your microscope compatible with the QIAscout?
Order Now

*All pictures shown here are for illustration purpose only.

Cell Purity

  • The release needle and the magnetic wand do not act as sources of contamination while isolating single cells using the QIAscout. This was verified using two approaches, qPCR and Pyrosequencing, after processing cells using the REPLI-g Single Cell Kit.

    Read App Note

    i
    No DNA contamination observed with the release needle in qPCR analysis. A. qPCR amplification plots of 15 microrafts pierced using unwashed release needle. B. qPCR amplification plots of negative controls (water). C. qPCR amplification plots of positive controls (10 ng DNA) showing a CT value of 27.
  • The release needle and the magnetic wand do not act as sources of contamination while isolating single cells using the QIAscout. This was verified using two approaches, qPCR and Pyrosequencing, after processing cells using the REPLI-g Single Cell Kit.

    Read App Note

    i
    No cell contamination observed with the magnetic wand in Pyrosequencing analysis. A. Representative pyrogram showing the G>A point mutation in the human EGFR gene in a sample with single SW48 cells (positive control). B. Representative pyrogram showing absence of the A allele in single HT-29 cells, transferred with the same unwashed magnetic wand as the SW48 cells. C. Frequency of the A allele in two pierced HT-29 cells (negative control), two pierced SW48 cells (positive control) and 24 pierced HT-29 cells. Single SW48 cells transferred using a clean magnetic wand (positive control) show a mean frequency of A allele (=point mutation) of 50.7%. Pierced single HT-29 cells transferred using an unwashed magnetic wand and analyzed for the presence of SW48 cell contamination showed a similar frequency of A allele when compared to the pierced single HT-29 cells transferred using the clean magnetic wand (negative control), indicating no contamination between the two cell lines, the arrays and the microrafts.

Cell Viability

  • Cells remain viable for several days and form subpopulations, irrespective of whether the pierced microrafts containing single cells are transferred to a cell culture dish or single cells present on microrafts are left intact and allowed to grow on the QIAscout array itself.

    Read App Note

    i
    Cells remain viable after piercing. A-B. Pierced microraft containing single SW48 cell. The microraft containing a colony of SW48 cells after several days of cultivation in a cell culture dish. C. Viable colony formed from a single MCF-7 cell. The position of the microraft in the cell culture dish is not important for further cultivation of pierced single cells.
  • Cells remain viable for several days and form subpopulations, irrespective of whether the pierced microrafts containing single cells are transferred to a cell culture dish or single cells present on microrafts are left intact and allowed to grow on the QIAscout array itself.

    Read App Note

    i
    Cells remain viable on the QIAscout array. Successful cultivation of a single HT-29 cell to form subcolonies on the QIAscout array after 90 hours confirms cell viability on the array.

Targeted Selection

  • The QIAscout enables efficient and reliable isolation of single targeted cells based on different cellular parameters like morphology or fluorescence.
    Conventional methods like pipetting and serial dilution are less successful in selective isolation of single cells when compared to the novel QIAscout method.

    Read App Note

    i
    A mixture consisting of three different cell lines (in the ratio of 1:1:1) was prepared containing one specific mutation per cell line:

    1) HT-29 (specific mutation: BRAF c.1799T>A)
    2) LoVo (specific mutation: APC c.3340C>T)
    3) MDA-MB-231 (red fluorescent cells, specific mutation: BRAF c.1391G>T)

    From this cell mixture, single fluorescent cells (MDA-MB-231) were targeted for isolation using three different methods: QIAscout, pipetting and serial dilution.
  • The QIAscout enables efficient and reliable isolation of single targeted cells based on different cellular parameters like morphology or fluorescence.
    Conventional methods like pipetting and serial dilution are less successful in selective isolation of single cells when compared to the novel QIAscout method.

    Read App Note

    i
    A mixture consisting of three different cell lines (in the ratio of 1:1:1) was prepared containing one specific mutation per cell line:

    1) HT-29 (round cells, specific mutation: BRAF c.1799T>A)
    2) LoVo (elongated cells, specific mutation:
    APC c.3340C>T)
    3) SW48 (round cells, specific mutation: EGFR c.2155G>A)

    From this cell mixture, single elongated cells (LoVo) were targeted for isolation using three different methods: QIAscout, pipetting and serial dilution.

miRNA Expression Profiling

  • Single-cell analysis for miRNA expression reveals heterogeneity across cell populations. In bulk cell analysis “cellular averages” mask intrinsic transcriptional variability. Distinct cell populations can be effectively revealed by single-cell analysis using the QIAscout for miRNA expression profiling.

    Read App Note

    i
    Principle Component Analysis of miRNA expression of single cells. Differential response in miRNA expression amongst treated single cells with 3 distinct populations.

Whole Genome Sequencing

  • Single cells isolated using the QIAscout demonstrate good sequencing metrics in downstream NGS analysis and can be used for the detection of subpopulation-specific SNPs using low-pass sequencing and the census-based variant calling method.

    Read App Note

    i
    Quality of sequencing run.

    86.3% bases in all reads have a Q Score of 30 and above, i.e., for the vast majority of bases the Inferred Base Call Accuracy is 99.9% with the probability of an incorrect base call being 1/1000.
  • Single cells isolated using the QIAscout demonstrate good sequencing metrics in downstream NGS analysis and can be used for the detection of subpopulation-specific SNPs using low-pass sequencing and the census-based variant calling method.

    Read App Note

    i
    Representative Pyrograms of bulk samples of LoVo and SW48 cells.

    Pyrosequencing analysis was used to validate the initial NGS results. The results confirmed the cell line specificity of the six SNPs as indicated by the NGS data.

Analysis of CTCs

  • Single circulating tumor cells (CTCs) that have been pre-enriched with the AdnaTest ProstateCancerSelect technology are suitable for isolation using the QIAscout system. Furthermore, the isolated single CTCs are compatible with downstream mRNA profiling using the AdnaTest ProstateCancerDetect.

    Read App Note

    i
    Results of AdnaTest ProstateCancerDetect.

    Expression pattern and transcript levels of three tumor markers (PSMA, PSA, EGFR) and one cell marker (actin) are shown.
    mRNA profiling was successful for all cells analyzed, as shown by the expression of actin in all samples. This indicates that the transfer rate of cells from the QIAscout array to the reaction tube was 100%.

Get in touch!

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What our customers say…

"QIAscout turned out to be a very suitable system allowing us to select and transfer our cell of interest with nearly 100% efficiency and survival rate for further downstream application."Dr. Lajos Kemeny, University of Szeged

"With the QIAscout, researchers will be able to control exactly which cell to spend analysis resources on, …"Christoph Ziegenhain, Ludwig-Maximilians University, Munich

"…overcoming bulk averaging of cell populations and learning more about the different cell types present in biological samples."Christoph Ziegenhain, Ludwig-Maximilians University, Munich