QIAcube Connect – redefining automated sample processing

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Connectivity

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Bar code reader

By scanning the 2D bar code on a QIAGEN kit box using the scanner, the kit information is transferred to the QIAcube Connect. Manual selection of the kit is not required.

Connectivity

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QIAcube Connect App

The QIAcube Connect App provides easy setup, eliminating the need for paper protocol sheets. Once the run has begun, the QIAcube Connect App provides real-time run updates, and sends notifications when a run is completed or an error has occurred. You can also use the app to create run reports. The Q-Base system enables seamless communication between the QIAcube Connect App and the instrument, to ensure that you are connected to your run and workflow wherever you are.

Virtual Prep

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Setup

With over 3000 protocols available, over 80 QIAGEN spin-column kits can be automated. This virtual prep demonstrates sample processing with QIAcube Connect.

You can select sample type, kit and starting material. The touchscreen displays the selected protocol and guides you through the instrument setup step-by-step.

Virtual Prep

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Load Check

The load check starts – the optical sensor checks the number and position of the loaded samples, and ensures that the correct number of rotor adaptors are loaded and positioned properly.

The ultrasonic sensor then checks that there is sufficient buffer for the protocol.

Finally, the optical sensor checks that the correct tip type and a sufficient number of tips is loaded. The QIAcube Connect will now start the protocol.

Virtual Prep

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Lysis

When the QIAcube Connect picks up a new tip, it passes it through a tip sensor to ensure that the correct tip type is used.

Lysis buffer is then added to each sample, and the filter tip is discarded.

The samples are incubated in the heatable shaker until lysis is complete.

Virtual Prep

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Bind

Lysates are applied to the spin columns in the centrifuge.

The centrifuge lid closes. The centrifugation step enables nucleic acids to bind to the spin-column membrane.

Virtual Prep

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Elute

The robotic gripper transfers each spin column from the wash to the elute position.

Elution buffer is applied to each spin column.

The centrifugation step enables elution of nucleic acids from the spin columns into an elution tube.

Run completed appears on the touchscreen and tablet confirming run completion.

Nucleic acid is ready for downstream applications.

QIAcube Connect – for fully automated sample processing using QIAGEN spin-column kits

UV Light

The UV light ensures effective worktable decontamination after the run.

Learn more about the QIAcube Connect UV-light decontamination procedure in this Application Note.

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What QIAcube* users say…

"We have two QIAcubes in our lab now and can't imagine life without them!"Lynne Shelley, Rochester, New York

"Not only do they decrease the hands-on time for sample prep, but they help eliminate the chance of human error."Lynne Shelley, Rochester, New York

"We'd never go back to manual extractions."Lynne Shelley, Rochester, New York

"Our QIAcube is such an essential part of our lab that he deserved a name, so his name is George."Joseph Washburn, University of Michigan

"He's a 'tech saver' and a time saver, allowing our technicians to prepare ahead of time, manage multiple tasks at once and use their overall time more wisely."Joseph Washburn, University of Michigan

* The widely used QIAcube – forerunner of the next-generation QIAcube Connect that is redefining the benefits of automating sample processing.