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Maximizing PCR and RT-PCR Success - Selection Guide applications

PCR Brochure 10/2016 23 Selection Guide QIAGEN multiplex end-point PCR solutions •: Recommended kit. * Up to 2 months. † Up to 6 months. Type-it Microsatellite PCR Kit Type-it Mutation Detect PCR Kit QIAGEN Multiplex PCR Kit QIAGEN Multiplex PCR Plus Kit Application Genotyping Any multiplex PCR application Starting material gDNA gDNA/cDNA Application STR and microsatellite analysis • • • Mutation detection • • • SNP loci amplification • • • Single-cell PCR • • Preamplification • • PCR performance Hot-start (15 min activation) • Hot-start (5 min activation) • • • Q-Solution (PCR enhancer for difficult templates) • • • • Maximum sensitivity and specificity • • • • Amplification-product size ≤0.5 kb ≤3.5 kb ≤3.5 kb ≤3.5 kb Ease of use and convenience PCR buffer with tracking dyes (CoralLoad) • • Room-temperature setup • • • • Fridge storage •* •* •† •* Master mix format, including nucleotides • • • •

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